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When cells encounter mycoplasma? Don't worry. This is it. Mycoplasma is gone

source:QiDa technoligy  views:182  time:2024-05-31

When cells encounter mycoplasma? Don't worry. This is it. Mycoplasma is gone

Mycoplasma (PPLO) is a small (0.2-0.3 micron) mencyless bacterium that can grow to very high concentrations, typically 107-108 organisms /ml, in mammalian cell cultures that are still not visible through conventional light microscopy. Using electron microscopy, it can be observed (figure below) that although late mycoplasma contamination causes the cell culture to become acidic, there are usually no obvious signs of contamination of the culture except as described below.


After magnification of mycoplasma:


Characteristics of mammalian cell cultures: In the early stages of contamination, mycoplasma usually does not cause changes in the pH of the medium and does not produce significant toxic effects on mammalian cells. The growth of mycoplasma is mainly related to the cell membrane of mammals. In many cases, there is no sign of mycoplasma contamination, however, mycoplasma can cause changes in cell growth characteristics, inhibition of cell metabolism, disruption of nucleic acid synthesis, chromosomal aberrations, changes in cell membrane antigenicity, and can alter transfection rates and viral acceptability.

The only way to confirm mycoplasma contamination is to perform routine tests using special techniques, as shown below:


Typical routes of infection in cultures: In most cases, mycoplasma contamination occurs through cross-contamination of untested infected cells with other cell lines. When more than one cell line is infected at a time, or when the same vial of medium is used for more than one cell line, the contamination usually begins to transfer to other cells through microscopic atomization in the air during pipetting or during routine treatment of the medium.

The best preventive measure is a combination of good aseptic techniques with routine testing. In the course of a day or week, in order to handle the culture, try to be "clean to dirty". That is, the confirmed uncontaminated cells are treated first, the unknown or untested cells are treated second, and the cells suspected or known to be contaminated but must remain in the laboratory for special reasons are treated last.

Antibiotics: Most conventional antibiotics used in cell culture are ineffective against mycoplasma. Gentamicin sulfate, kanamycin sulfate, and tylomycin tartrate are effective to some extent, but may only be inhibitory, not mycoplasticidal,

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