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No Trifles In Scientific Research (I) STR Identification Of Cells

source:Qida organism  views:1772  time:2020-10-20

  & Nbsp; When Doing Experiments, We Found That There Were Too Many Kinds Of Cells, So We Need To Consult Various Literatures To See The Characteristics Of Each Cell, So As To Design The Experimental Plan And Price. We Found That We Need Cell Identification When We Submitted Articles After Completing The Experiment With Compact Experimental Types& Nbsp; & Nbsp; According To Statistics, About 30% Of Cell Lines Were Cross-contaminated Or Misidentified, And The Use Of Cross-contaminated Or Misidentified Cells Led To Incorrect Research Conclusions, Unrepeatable Results, And Catastrophic Consequences Of Clinical Cell Therapy... This Wastes A Lot Of Time, Energy And Money. Therefore, NIH, ATCC And Other Authoritative Institutions Have Repeatedly Called For Researchers To Identify Cells. Recently, American Media Reported That 1/6 Of Scientists Are Using "fake" Cells In Their Research. In December 2014 And February 2015, Science Magazine Published Articles On The Seriousness Of Cross Contamination And Wrong Identification Of Cells; In April 2015, Nature Informed That All Journals Under Nature Would Require The Authors To Identify The Cell Lines Used In The Paper; In June 2015, It Was Reported That A Scientist Withdrew His Nature Paper Due To The Wrong Cell Line. NIH, ATCC, Nature, Science And Other Institutions Have Repeatedly Called For Researchers To Identify Cells. STR Genotyping Has Been Used As The Gold Standard For Cell Line Identification. More And More Journals Have Begun To Require Contributors To Provide Cell STR Identification Results& Nbsp; & Nbsp; In 2011, The United States Has Issued The National Standard For Cell STR Identification (ANSI/ATC ASN-0002-2011). At Present, STR Detection Has Been Widely Used In Parent-child Identification, Individual Identification, Cell Origin Determination, Etc. ATCC, DSMZ, JCRB And Other International Well-known Cell Banks Have Listed STR Genotyping As The Gold Standard For Cell Identification& Nbsp; & Nbsp; STR Identification Has Become The "ID Card" Of Cell Lines, Which Can Pass Freely In The Scientific Research Field, And Must Have STR. As For Animal Origin, Because The Established DNA Bands Are Not Perfect, Only Some Cells Of Mice Can Provide STR At Present. STR Identification Description: 9/16 STR Loci Of Cell Samples Were Genotyped By Fluorescent Labeled Amplification Product Length Polymorphism Analysis. PCR Primers Were Designed To Form Two PANELS For Amplification Of Polymorphic Sites. The 5 'FAM Fluorescent Marker On Primer Was Used To Label The Site. Software Design Of Primer 3 Online( Http://frodo.wi.mit.edu/cgi-bin/primer3/primer3_www.cgi )。 After Diluting The PCR Product, Take A Small Amount And Mix It With The Internal Standard Label, And Directly Put It On ABI 3130xl For Capillary Electrophoresis. The Data Files Are Analyzed With GeneMapper 4.0 (Applied Biosystems)& Nbsp; & Nbsp; STR (Short Tandem Repeat) Is A Kind Of DNA Tandem Repeat Sequence That Widely Exists In Eukaryotic Genomes. Due To The Individual Difference Polymorphism Of Its Core Sequence Repeats, STR Is Also Called The DNA Fingerprint Of Cells. Our Sample Collection And Identification: 1. T25 Bottles Of Live Cells And Cryotubes May × The Cell Suspension Above The Fifth Power Of 10 Is Placed In A 1.5ml EP Tube, Sealed With A Sealing Membrane, Marked And Attached With A List. 2. Identification Time: Provide Results And Identification Report Within 2 Weeks.
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