No Trivia In Cell Culture (II) How To Select Serum

The Cells Cultured In Vitro Live Directly In The Culture Medium, So The Culture Medium Needs To Meet The Requirements Of The Cells On Nutrients, Growth Promoting Factors, Hormones, Osmotic Pressure, PH And Other Aspects; How To Choose The Culture Medium Has Become A Key Problem During The Primary Extraction. In Addition, Serum Can Simulate The Living Environment Of Individuals During The Cell Extraction, So Serum Is Essential When Extracting Cells. The Nutritional Conditions To Maintain Cell Growth Generally Include The Following Aspects: Amino Acids, Monosaccharides, Vitamins, Inorganic Ions And Trace Elements, In Addition To Growth Promoting Hormone, Osmotic Pressure, PH Value, And To Ensure Non-toxic And Pollution-free Reagent Preparation. The Normal Medium For Cell Culture Is Nothing More Than Basic Medium Plus Serum. Serum Is A Mysterious Reagent. Up To Now, It Plays An Important And Even Irreplaceable Role In Cell Growth And Reproduction& Nbsp; Main Components Of Serum: Serum Is A Very Complex Mixture Formed By Removing Fibrin From Plasma. Although Most Of Its Components Are Known, There Are Still Some Unknown, And The Composition And Content Of Serum Often Vary With The Sex, Age, Physiological And Nutritional Conditions Of Blood Supplying Animals. Serum Contains Various Plasma Proteins, Polypeptides, Fats, Carbohydrates, Growth Factors, Hormones, Inorganic Substances, Etc. The Main Function Of Serum Is To Provide Basic Nutrients: Amino Acids, Vitamins, Inorganic Substances, Lipid Substances, Nucleic Acid Derivatives, Etc. It Is Necessary For Cell Growth. The Criteria For Judging Serum Are As Follows: A Good Serum Should Be Transparent, Clear, Yellowish Brown Or Yellowish Brown, With No Or Very Little Precipitation, And Relatively Thick. If The Serum Is Turbid, Opaque And Contains Many Precipitates, It Indicates That The Serum Is Polluted Or The Protein In The Serum Is Denatured; Generally, When The Serum Is Repeatedly Frozen And Thawed Or Contaminated With Mycoplasma, There Will Be A Lot Of Serum Precipitation. If The Serum Is Brownish Red, It Indicates That The Hemoglobin Content In The Serum Is Too High, And Hemolysis Occurs When The Material Is Taken; If The Liquid Is Thin When Shaking, It Indicates That There Is Too Much Normal Saline Mixed In The Serum. If You Want To Further Understand The Quality Of Serum, You Should Continuously Cultivate Some Cells And Observe The Growth Of Cells. It Is Recommended To Inactivate The Serum Of Average Quality When Using, That Is, At 56 ℃ For 30 Minutes. This Is Mainly To Prevent The Complement Components In The Serum From Causing Toxicity To Cells. The General Serum Does Not Need To Be Inactivated, Because Heating Will Also Lead To The Loss Of Some Stimulators And Proteins, Which Is Not Conducive To Cell Growth& Nbsp; The Serum Is Generally Stored At - 20 ℃, And Repeated Freezing And Thawing Should Be Avoided. Repeated Freezing And Thawing Will Lead To The Release Of Protein In The Serum, Which Will Lead To The Increase Of Precipitation. After Purchasing Large Packages Of Serum, First Inactivate Them, Then Sub Package Them Into Small Packages, Store Them At - 20 ℃, And Slowly Thaw Them 4 Degrees Before Use. Different Batches Of Serum Will Have Great Differences. In Order To Make The Experiment Uniform And Repeatable, It Is Recommended To Ask For A Trial Kit For Testing Before Purchasing Serum. After Selecting A Batch, It Is Necessary To Retain Enough For Use For 6 Months To 1 Year Until Another Batch Of Pre Tested Samples Is Used To Replace It.