Mouse Immortalized Conditioned Bone Cell Line (IDG-SW3)

Background: IDG-SW3 Representative A Non Residential Population Progressing From Early Osteoblasts To Late Osteocellular These Cells Express Functional SV40 Large T Antigen That Is Induced In The Presence Of IFN  γ  Under Permissive Temperature (33°C) And Express GFP Under Control Of The Dentin Matrix (Dmp1) Promoter.  Initial Culture Shows Osteoblastic Phenotype, However When Under Mineralizing Conditions, The Cells Start To Express Early Osteocyte Markers Such As E11/podoplanin, Followed By Dmp1 And Mature Markers SOST And FGF23 By 21-28 Days Of Culture.& Nbsp; Similar To Osteocytes In Vivo, These Cells Respond To Hormonal Signals Such As PTH And 1,25-dihydroxyvitamin-D3.  IDG-SW3 Cells Can Be Maintained In Both 2D And 3D Cultures And Are Useful For Study Of Osteocytes At Various Differentiation Stages.  It Should Be Noted That These Cultures May Contain A Mixture Of Osteoblasts And Osteoblasts At Different Stages Of Differentiation And For Studies Requiring Enriched Osteoblasts, The Dmp1-GFP Cells Should Be Isolated By Flow Cytometry (FACS). These Cells Expressed Functional SV40 Large T Antigen, Which Was Induced At The Allowable Temperature (33 C) Without IFN Expression, And Expressed GFP Under The Control Of The Dentin Matrix (Dmp1) Promoter. Initial Culture Showed Osteoblast Phenotype, But Under Mineralized Conditions, Cells Began To Express Early Osteoblast Markers, Such As E11/podoplanin. After 21-28 Days Of Culture, Cells Expressed Dmp1 And Mature Markers SOST And FGF23. Similar To Bone Cells In Vivo, These Cells Respond To Hormone Signals Such As PTH And 1,25-dihydroxyvitamin D3. IDG-SW3 Cells Can Be Maintained In 2D And 3D Culture, Which Is Very Useful For Studying Osteoblasts At Different Differentiation Stages. It Is Worth Noting That These Cultures May Contain A Mixture Of Osteoblasts And Osteoblasts At Different Stages Of Differentiation. For Studies That Need To Enrich Osteoblasts, Dmp1-GFP Cells Should Be Separated By Flow Cytometry (FACS). Animal Species/Oganism & Nbsp; Mouse Tissue Source; Tissue And Cell Type  Osteoblast Morphology; Wall Attached Growth& Nbsp; Note: This Product Is Only For Scientific Research