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Current position:Home > Products > highQu GmbH > PCR & qPCR > Standard & Direct PCR > UDGin™ PCR nucleic acid air soluble contamination scavenger
  • UDGin™ PCR核酸气溶污染清除剂

UDGin™ PCR nucleic acid air soluble contamination scavenger

No.:UDG0101

Price: ¥686.00

specification:
500 r of 20 µl
amount: - +
  • UDGin™ PCR核酸气溶污染清除剂
Products Description

UDGin ™ PCR Cleaner Mix is an effective tool to prevent contamination in qPCR or PCR. It is optimized for use with highQu qPCR or PCR products.

UDGin ™ PCR Cleaner Mix can use the best combination of 20X uracil - dna glycosylase (UDG) and dUTP in stabilizer buffer. 1µl UDGin ™ The addition of PCR Cleaner Mix to µ l PCR reaction in every 20 samples can prevent the DNA amplification carried by dUTP PCR.

In the incubation process 10 minutes before the start of PCR, UDG hydrolyzes the n - glycosidic bond between uracil and sugar, leaving pyrimidine-free sites on the uracil containing DNA, and then in the first PCR cycle, these sites are thermally sheared, so only the newly added template is amplified. UDG is inactivated during the initial PCR denaturation process without destroying the newly synthesized dUTP containing dna. Use UDGin ™ The PCR products obtained by PCR Cleaner Mix contain uracil, so they will be destroyed by UDG again before the next PCR.

Product application:

Effective prevention of residual contamination in qPCR

Study on effective prevention of carry-contamination in PCR

Product advantages:

Mixing with UDG and dUTP can save time

Compatible with all highQu qPCR and PCR main mixtures




Statement: The product is only used for scientific research. The use of UDG for certain applications in some countries may involve patents and may require licensing.


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