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Tumorigenecity: Yes, in nude mice and also in hamster cheekIsoenzyme: Me-2, 2; PGM3, 1-2;PGM1, 1;ES D, 2;AK1, 1;GLO-1, 1-2;G6PD, B.Histopathology: Neuroblastoma, metastasis to supra-orbital areaSubculture: Remove medium, rinse with fresh 0.25 % Trypsin solution, remove trypsin and let the culture sit at room temperature(or at 37'C) until the cells detach(about 10 minutes). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days.Split ratio: Media change: Reverse transcritase: Production.