RBL-2H3

Tumorigenecity: Isoenzyme: Histopathology: leukemia, chemically inducedSubculture: Remove spent medium, add fresh 0.25% Trypsin, 0.03% EDTA solution, rinse and remove trypsin. Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.Split ratio: 1: 4 to 1: 8Media change: every 2 to 3 daysReverse transcritase: Productionhistamine.

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