4T1-GFP

Mouse 4T1 is a 2-Amino-6-purinethiol resistant cell line selected from the 410.4 tumor without mulagen treatment. RMPI-1640 containing 10% serum is recommended for 4T1 cell expansion and these cells have a guaranteed average>50 additional population doubling levels when ciltured following the detailed protocol described below. GFP-expressing 4T1 cells are puromycin resistant cells after transfected with lentiviruses expressing GFP under the control of CMV promotoers.

For research use only