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Never allow cultures to become completely confluent before subculture. The serum used is calf serum, Not FBS. The serum initially employed and found satisfactory was from the Colorado Serum Co.; Tested and found negative for ectromelia virus(mouse pox).Tumorigenecity: noIsoenzyme: Histopathology: Subculture: Split ratio: Media change: Reverse transcritase: negativeProduction.