A-704-

Human kidney adenocarcinoma. Derived from 78-year-old male. Does not produce tumours in nude mice. Tumorigenecity: No. Isoenzyme: Me-2, 1;PGM3.1-2;PGM1, 1;ES-D, 1;AK-1, 1;GLO-1, 2;G6PD, B; Histopathology: adenocarcinoma; Subculture: Remove medium, rinse with fresh 0.25% Trypsin solution, remove trypsin and let the culture sit at room temperature (or at 37) until the cells detach (about 10 min). Add fresh medium, aspirate and dispense into new flasks. Subculture every 6 to 8 days. Split ratio: 1: 3 to 1: 4; Media change: 2 to 3 times weekly; Reverse transcritase: Production.

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