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Simple And Practical, Extraction Of Umbilical Cord Mesenchymal Stem Cells

source:Qida organism  views:2015  time:2022-09-16

Mesenchymal Stem Cells Exist In Many Organs And Tissues Of The Human Body. They Have The Potential To Regenerate And Differentiate, And Play An Important Role In Repairing Damaged Tissues In The Body. Adult Mesenchymal Stem Cells Have Been Identified In Bone Marrow, Fat, Blood, Intestine, Skin, Muscle, Umbilical Cord And Other Tissues. Their Number Is Limited, Isolation Involves Invasion, Inducing Donor Site Diseases, Donor Heterogeneity And Other Shortcomings, These Problems Make Researchers Interested In The Research Of Perinatal Stem Cells. Let's See How To Extract Umbilical Cord Mesenchymal Stem Cells. 1. Take Healthy And Natural Fetal Umbilical Cord And Cut It Into 3cm Segments. Then Wash The Residual Blood With PBS For Several Times, Remove Two Arteries And One Vein, And Cut It Into Small Pieces Of About Imm3. 2. Add PBS For Cleaning, Centrifugate At 800rmp For 5 Minutes To Remove The Supernatant, And Clean Twice. 3. Add 20ml Type I Collagenase To Digest For 2h. During The Digestion Process, Stop Mixing Tissue Blocks To Promote Digestion. 4. After Digestion, Remove The Supernatant By Centrifugation At 800rmp For 5min, Add PBS For Cleaning, And Centrifugate At 800rmp For 5min For Cleaning Twice. 5. The Digested Tissue Block Was Transferred To 3 Bottles Of 25ml Culture Bottle Containing 5ml Culture Medium And Put Into The Incubator At 37 ℃ And 5% C02 For Culture. 6. Do Not Move The Culture Bottle For 3 Days To Make The Tissue Block Stick To The Wall. Change The Liquid Half On The Fourth Day, And Change The Liquid Full On The Seventh Day. The Cell Growth On The Twelfth Day Is As Follows:; When The Overall Confluence Is Above 80%, 1:3 Passage Will Be Carried Out, And Some Cells Will Pass Into The Orifice Plate For Immunofluorescence Detection, As Shown In The Following Figure:& Nbsp;& Nbsp;& Nbsp; CD-90 Immunofluorescence Detection& Nbsp; CD-29 Immunofluorescence Detection; Humsc Cells Are Generally Identified By Immunofluorescence In P1 Generation, And Can Be Frozen In P2 Generation. Cells Can Be Multiplied By More Than 15 Times Using MSCM Low Serum Stem Cell Culture Medium. MSCM Medium Growth Factor Contains Components That Promote Stem Cell Growth And Small Molecular Compounds That Inhibit The Growth Of Mixed Cells. When Cells Are Separated Using MSCM Culture Medium, The Serum Needs To Be Increased To 10%& Nbsp;& Nbsp; From September 16, Anyone Who Buys A 500ml Series Of MSCM Stem Cell Culture Media (Qida Biology, Product No.: P2001/P2005) Will Be Given A Humsc Cell Discount Coupon Of 500 Yuan, And Two Sets Will Be Given A Humsc Cell Discount Coupon Of 1000 Yuan, And So On. If You Buy More, You Will Be Given More. If You Buy 10 Sets At A Time, You Will Be Given A Humsc Cell Free Of Charge.
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