It's Easy To Become Tumor In Nude Mice

Cell Derived Xenograft (CDX), That Is, The Tumor Cells Subcultured In Vitro Are Inoculated Into Immune Deficient Mice. The Subcutaneous Tumor Bearing Nude Mice Refers To The Injection Of Human Or Mouse Derived Cells Into The Subcutaneous Tissue Of Immune Deficient Nude Mice To Verify The Formation, Development Of Tumors And The Effect Of Anti-tumor Drugs Through Other Conditions& Nbsp; Tumor Cells Can Be Benign Or Malignant; Cancer Cells Are Malignant And Can Spread. Tumor Cells Contain Cancer Cells, And Their Common Feature Is That They Are Out Of Control From The Gene Level And Can Proliferate In A Limited Way. Cancer Cells Have Three Significant Basic Characteristics: Immortality, Mobility And No Contact Inhibition Reaction& Nbsp; Conventional Cells Easy To Form Tumors: Hep-G2 (liver Cancer), NCI-H460 (lung Cancer With Lung Cancer Cells), 97H (human Liver Cancer Cells); 4T1 (mouse Breast Cancer) SK-OV-3 (ovarian Cancer), MCF-7 (breast Cancer) SKBR3, A375/B16-F10 (⼈⿊⾊ Tumor Cell), H1299 (lung Cancer), SPC-A-1 (lung Adenocarcinoma), HT-29/HCT116 (colon Cancer) MDA-MB-231 Breast Cancer, CT26 (⼩⿏ Colon Cancer Cell), U87 (⼈ Glioma Cell), PC-3 (⼈ Prostate Cancer Cell), Etc; Common Mouse Phenotypes: & Nbsp; Selection Of Tumor Bearing Nude Mice: 4-6 Week Old BALB/c-nu Nude Mice; Operating Steps: 1. Take The Cells In Logarithmic Long-term, With The Cell Density Of About 80-90%. 2. Wash The Cells With Pre Cooled PBS For 1-2 Times After Trypsin Digestion To Remove The Residual ⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎⾎121. 3. Suitable Concentration Of PBS Or 0.85% Normal Saline Blowing Cells To Precipitate, And The Number Of Cells Inoculated With Sub Tumor Is 1-5 × 106 Cells/⽀, The Inoculation Volume Is 0.1-0.2ml, So The Concentration Of Cell Suspension Is 1-5 × 107 Cells/ml. After Digestion, The Cells Should Be Inoculated Under The Naked ⿏⽪⽪⼀⼀⼩⼩⼩⼩⼩⼩⼩⼩⼩⼩ As Soon As Possible (if The Inoculation Amount ⼤⼤⼤⼤⼤⼩⼩⼩⼩⼩12073& Nbsp; 4. Grasp The Mouse's Tail (away From The Tail End), Lift The Mouse, Grasp Its Front Foot, And Pinch The Skin On The Back Of The Naked Neck With The Left Thumb And Fingers, And Clamp The Naked Tail With The Left Thumb; For The Convenience Of Injection, The Injection Planting Site Should Be Selected In The Area With Abundant Supply, Such As The Middle And Rear Part Of The Armpit. If Bitten, Do Not Drop The Animal From Your Hand, Because Falling Will Hurt The Animal And Lead To Its Death, As Well As Possible Bacterial And Viral Contamination In The Future. The Best Way To Remove The Mouse From Your Finger Is To Put Your Hand Back Into The Cage. This Provides A Way For The Mouse To Go Home, And It Will Jump Off Your Finger And Return To The Cage. 5.  The Needle Gun Will Blow The Cell Suspension Fully And Then Inoculate It. During The Inoculation, The Needle Will Enter The Needle Deep Point Under The Needle, About 1cm Deep. The Needle Will Slide Left And Right Under The Needle For Several Times, So That The Cells Can Be Inoculated Into Clusters And The Cell Suspension Will Not Overflow From The Needle Eye After Injection. 6.  According To The Characteristics Of Tumor Cells And The Amount Of Cells Inoculated, The Tumorigenesis Cycle Under The General Tumor Is 1-2. The Tumor Should Not Be Longer Than ⼤⼤⼀⼀⼀⼀⼀⼤⼩⼩⼩⼀⼩⼩⼩⼀⼩⼀⼤⼩⼩⼩⼩⼀⼀⼀⼀. V=1/2ab2 (a Is The Major Axis, B Is The Minor Axis); The Problems That Nude Mice May Encounter With Tumor Bearing: 1. Does The Tumor Grow Up And Disappear? It Is Very Common For Tumor Lump To Shrink First And Then Change Later. It May Be An Inflammatory Reaction. The Tumor Cells Are Absorbed By The Tumor Cells. Continue To Observe Whether The Tumor Cells Can Grow After The Absorption Of The Tumor Cells. If They Cannot Grow, It Is Recommended To Replace Them; 2. Is It Possible To Inoculate At Multiple Points In The Hospital? No, Only ⽼⿏⼀⽼⿏ Can Only Inoculate ⼀ Points, Because If There Are Multiple Tumor Blocks On ⼀⽼⿏⾝, They May Affect Each Other& Nbsp; 3. What's Wrong With The Tumor Failing To Grow? ⼀⽣⽣⽣⽣⽣⽣⽣⼀⽣⼀⼀⽣⼀⽣⼀⼀⼀⽣⽣⽉⽉⼤⼤⼤⽉; ⼆: It May Be That The Cells Are In Poor Condition And Live Poorly, Or The Tumor Cells Are Not Easy To Form Tumors, So It Is Necessary To Add ⼊ Matrix Glue (matrix Glue: PBS=1:1) Or Increase ⼤ Inoculation Amount Or Replace The Cell Line; 3： ⼀ ⽅ ⾯ May Be ⼩ ⿏ Weeks Old, Or ⼩ Immune Rejection. It Is Recommended To Replace ⼩ (Nod Scid ⼩ ⿏) With More Severe Immune Deficiency& Nbsp; 4. The Difference In Tumor Volume Is Very Large, But Not Large? First Of All, Not All Tumors Can Grow In Uniform Shape. Secondly, This May Be Caused By The Age Of The Pig And The Lack Of Inoculated Cells. It Is Recommended To Re Inoculate& Nbsp; 5.  Does The Subcutaneous Or Intradermal Tumor Grow Rapidly, Or Appear Ulcer And Necrosis After Growing To A Certain Size? Considering The Cause Of Small Animal Infection, We Can Provide Small Animals With Antibiotics To Support Treatment And Prevent Infection. Antibiotics (and Other Drugs) Can Be Used In Small Animal Drinking Water For The Following Reasons: - Stability Of Drugs In Water- The Mice Were Treated By Drinking Water. If Necrosis And Ulcers Occur In The Whole Body Of Small Animals As Part Of The Subcutaneous Or Intradermal Natural Growth Mode, And The Length Is Less Than 1.5 Cm, Resulting In Skin Rupture Or Exudation (liquid Or Blood Accumulation) Lasting For More Than 48-72 Hours Without Closing The "wound", Euthanasia Treatment Is Usually Required For Small Animals.