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MOI Value Of Lentivirus Infection

source:Qida organism  views:2926  time:2022-07-15

When Cells Are Infected, How To Calculate The MOI Value? If More Cells Are Added, They Are Easy To Die. If The Concentration Is Not Enough, The Cells Will Not Be Infected. This Is A Headache. The Following Is An Absolute Collection Of Soft Articles About The Calculation Of The MIO Value Of Viruses. When Added To The Collection, Cell Transfection Can Be Checked At Any Time: First, What Is The Following MOI? MOI Is The Abbreviation Of "Multiplicity Of Infection", Which Is Translated Into The Plural Of Infection Or Infection Index, Meaning The Ratio Of Lentivirus And Cell Number When Successfully Infected. In The Experiment, We Also Defined The MOI Value Required For A Cell To Achieve 80% Infection Efficiency As The MOI Value Of The Cell. The MOI Index Can Be Found In Relevant Literature, The Following Is A Reference List Of MOI Values Of Qida Cells: (MOI Values Are Related To Cell Generation And Vitality) Qida Article Number Product Name Chinese Name Price MOI Value CD0092A431 Human Skin Squamous Cell Carcinoma Cell 14005CD0012A549 Human Non-small Cell Lung Cancer Cell 11005CD0447B16-F0 Mouse Skin Melanoma Cell 14006CD0062BxPC-3 Human In Situ Pancreatic Adenocarcinoma Cell 110010CD0039HCT116 Human Colon Cancer Cell 13005CD0094HeLa Human Cervical Cancer Cell 11003CD0021293 Human Embryonic Kidney Epithelial Cell 10006CD0180Hepa1-6 Mouse Liver Cancer Cell 12003CD0587HCMEC/D3 Human Brain Microvascular Endothelial Cell 150060CD0040HT-29 Human Colon Cancer Cell 13003CD0290HUVEC Human Umbilical Vein Endothelial Cell 2500100CD0114Jurkat Human Acute T-lymphocyte Leukemia Cell 150010CD0209LLC Mouse Lewis Lung Cancer Cell 9006CD0088LNCaP Human Prostate Cancer Cell 15005CD0052MCF-7 Human Breast Cancer Cell 14002CD0053MDA-MB-231 Human Breast Cancer Cell 14001CD0013MRC-5 Human Embryonic Lung Cell 14001CD0297NB4 Human Leukemia Cell 170010CD0156PC12  Rat Adrenal Medullary Chromaffin Tumor Differentiation Cell 120020CD0032SKOV-3 Human Ovarian Cancer Cell 140015CD0066U-2OS Human Osteosarcoma Cell 11005& Nbsp; Factors That Determine The Dosage Of Lentivirus Particles: Lentivirus Titer, MOI, Infection Volume, Cell Density (fusion Degree); How Many Lentiviruses Should Be Used For MOI Of Known Target Cells& Nbsp;& Nbsp;& Nbsp;& Nbsp; One × The Volume And Virus Amount Of The Culture Medium Used For 108 TU/mL Virus Infected Cells Are Referred To. Take 293T Cells As An Example: The Number Of Cells Per Well In The Culture Container Is Normal. When Infected, The Volume Of Lentivirus MOI=1, The Amount Of Lentivirus MOI=10, The Amount Of Lentivirus MOI=A, The Amount Of Lentivirus 96-well2.38 × 10^4100 µL100ul0.2ul2.4ul0.24*A Ul48-well4.75 × 10^4200 µL200ul0.5ul4.8ul0.48A Ul24-well9.5 × 10^4400 µL400ul1.0ul9.5ul0.85*A Ul12-well1.9 × 10^51ml500ul1.8ul19ul1.9*A Ul6-well4.75 × 10^52ml1ml4.8ul47.5ul4.75*A UlT25 Flask1.19 X 10^65ml1.5ml11.5ul118.5ul11.85*A Ul  Different Cell Types And Experimental Operations Are Different. It Is Suggested To Use Cell Counting Method To Calculate The Number Of Cells In Each Hole. The Degree Of Cell Confluence In The Cell Seed Plate Is 20-30%, And It Is Generally Appropriate To Grow To 80% - 90% Of The Fusion Degree After 3 Days Of Infection. Usually, Lentivirus Particles On The Upper Layer Of The Solution Are Difficult To Approach Cells. Reducing The Infection Volume Of The Medium Can Improve The Efficiency Of Virus Infection. After 1 Day Of Culture, Add The Culture Medium To Normal Volume. However, When The Cell Plate Hole Is Small, If The Infection Volume Is Halved, The Surface Tension Of The Liquid Will Cause The Distribution Of Lentivirus Particles To Be Uneven, So The Infection Volume Of The Small Hole Culture Plate Is The Same As The Normal Culture Volume.
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