Biological Characteristics Of Prostate Cancer Cell Lines

Prostate Cancer (PCa) Is The Most Common Malignant Tumor. Despite The Continuous Improvement Of Living Standards And Modern Medical Technology, PCa Is Still The First Threat To Men's Health;, From 2011 To 2015, The Total Incidence Rate Of Prostate Cancer Decreased By 7% Every Year. Therefore, It Is Important To Master The Biological Characteristics Of Common Prostate Cancer Cells LNCaP, PC3 And 22Rv1, Vcap, DU145.RM-1 And Other Cells Are Commonly Used Cell Lines In Prostate Cancer Research. The Following Is The Culture Method And Gene Expression Of Prostate Cancer Cells We Sorted Out. Remember To Collect& Nbsp; Name Source/Morphology Culture Medium Cells Expressing LNCaP Clone FGC Prostate Cancer Cells; Prostate/epithelial Cell Like Medium: 90% 1640+10% FBS+G/A Complete Medium: 1640 Complete Medium (product No.: MD0201) Gas Phase: 37 ℃, 5% CO2. Cell Growth Is Slow, Passage And Digestion. Please Note That Cells Should Be Allowed To Be Cultured Without Interference Within The First 48 Hours After Passage Culture. When Living Cells Are Transported, They Will All Float In The Culture Medium. Please Centrifuge 300 X G For 15 Minutes, And Then Suspend Them In 10 ML Culture Medium In 1:2 Bottles. Cells Can Form Tumor In Nude Mice;. Cell Pair 5- α- Dihydrotestosterone (growth Regulation And Acid Phosphatase Production) Reacts With Androgen Receptor (++); Estrogen Receptor (--), PC-3 Prostate Cancer Cell& Nbsp; Prostate/epithelial Cell-like Cells Grow In Agar And Generate Tumors In Nude Mice. Acid Phosphatase And Testosterone - 5- α Reductase Activity Is Low. 22Rv1 Prostate Cancer Cells; Prostate/epithelial Cell Like Gas Phase: Air, 95%; CO2，5%。 Temperature: 37 ℃. Tumor Formation In Nude Mice, PSA (+). Growth Was Weakly Stimulated By Dioxy Testosterone. Western Blot Analysis Showed That The Lysate Had An Immune Reaction With The Androgen Receptor Antibody. Epidermal Growth Factor (EGF) Stimulates Growth, But Transforms Growth Factor β- 1（TGF β- 1) Does Not Inhibit Growth. 22Rv1 Prostate Cancer Cells Can Produce High Titer Of Human Retrovirus XMRV (heterotropic Mouse Leukemia Virus Associated Virus) Vcap Prostate Cancer Cells Prostate/epithelial Cell Like Gas Phase: 37 ℃, 5% CO2 Cells Grow Slowly Cytokatin-189 (+), RB (+) & Nbsp;& Nbsp;& Nbsp;& Nbsp;& Nbsp;& Nbsp;; P53（+） ,PSA(+),PAP（+）； Tumor Was Formed In Nude Mice And SCID Mice, And VCaP Prostate Cancer Cells Produced Mouse Xenogeneic Retrovirus Bxv-1, Which May Be DU145 Prostate Cancer Cells Obtained By These Cells During Xenotransplantation In Mice Prostate/epithelial Cell Like Gas Phase: Air, 95%; CO2，5%。 Temperature: 37 ℃. Blood Type O; Rh+； Adenocarcinoma (Grade II) Was Formed In Nude Mice, Which Was Consistent With The Primary Prostate Cancer. The Cells Were Insensitive To Hormones And Weakly Positive For Acid Phosphatase. The Isolated Cells Formed Colonies In Soft Agar. DU145 Expresses Prostate Antigen. Ultrastructural Analysis Of Cell Lines And Primary Tumors Showed That Microvilli, Tensiles, Desmosomes, Mitochondria, Well Developed Golgi Bodies And Heterolysosome RM-1 Prostate Cancer Cells; Prostate/epithelial Cell Like Gas Phase: At 37 ℃, 5% CO2 Was Used To Infect The Urogenital Sinus Cells Of 17 Day Old C57BL/6 Embryos With Zipras/myc9 Retrovirus, And Transplanted Them Into The Renal Capsule Of Isogenic Adult Male Mice.