How To Deal With Live Cells?

  After Receiving The Cells, I Found That It Was A Culture Bottle, And I Was Helpless For The First Time. What Should I Do& Nbsp; First Of All, We Have To Figure Out Whether Our Cells Are Adherent Cells, Suspended Cells Or Semi Suspended Cells. For Those Who Do Not Understand, Please Refer To The Following Figure:; Adherent Cells: SKOV3& Nbsp;& Nbsp;& Nbsp; Suspended Cells: THP-1 Semi Suspended Semi Adherent Cells: BV-2 Cells; Adherent Cells Should Follow The Following Guidelines: After Receiving The Cells, Observe Them Under The Microscope. Some Cells Adhere Firmly To The Wall, And They Will Not Change Due To Transportation Problems. After Receiving The Cells, Observe The Cell Density Under The Microscope To Ensure That There Is No Pollution. If There Is No Abnormality, Put The Cells In The Incubator And Stabilize Them For 1-2 Hours, And Then Digest Them According To The Digestion Sequence Of Adherent Cells: I The Digestion Sequence Of Adherent Cells Is As Follows: 1. Preparation, Trypsin Preheating, Medium Preheating, PBS Preheating For 10-15 Minutes 1 Remove The Supernatant And Wash It Twice With PBS. 2. Discard PBS, Add 1ml Of Trypsin, Shake It Gently From Front To Back, Left To Right, And Place It On The Ultraclean Table At Room Temperature For About 3-5 Minutes. 3. Observe The Cells Under The Microscope. If The Cells Become Oval And Bright, Pat The Cell Bottle Wall Gently; 4. Observe Whether The Cells Have Fallen Off Under The Microscope. If They Do Not Fall Off, Continue Digestion For 2 Minutes And Then Gently Pat Them. If More Than 95% Of The Cells Fall Off, Immediately Add The Medium Containing Serum To Stop Digestion. 5. Transfer The Digested Cell Suspension Into A Centrifuge Tube For Centrifugation. Normal Centrifugation Speed: 1000-1200 Revolutions, 5 Minutes. 6. Separate Bottles. For The First Passage, It Is Recommended To Pass It 1:2, Or Pass It In A Bottle And Freeze One& Nbsp;& Nbsp; 2、 The Rules For Passage Of Suspension Cells: 1. After Receiving The Cells And Stabilizing Them For One Hour, Divide Them Into Two 50ml Centrifuge Tubes For Equal Centrifugation For 1000 Turns For 5 Minutes; 2. & Nbsp; Discard The Supernatant. One Tube Is Divided Into Two T25 Bottles For Subculture. One Tube Of Frozen Suspended Cells Does Not Need To Be Centrifuged Every Time For Subculture. It Is Recommended To Centrifugate Once Every Two Subcultures To Remove The Fragments In The Cells & Nbsp;& Nbsp; 3、 Digestive Essentials Of Semi Suspended And Semi Adherent Cells: 1. After Receiving The Cells And Stabilizing For One Hour, The Suspended Cells Are Collected And Centrifuged, And Adherent Cells Are Digested According To The Adherent Order & Nbsp; After Receiving The Cells, Please Read The Instructions Carefully And Operate According To The Passage Procedure. Please Keep The Same Culture Method As The Business. For Example, Cattle Eat Grass. If You Want To Improve Its Nutrition, You Can Only Improve The Quality Of The Grass. Never Replace It With Meat. Not Only Is It Expensive, But It Will Starve The Cattle To Death& Nbsp;& Nbsp;