Expression Genes And Culture Of Several Common Breast Cancer Cells

Breast Cancer Is One Of The Most Common Malignant Tumors In Women. Due To Different Geographical Environment And Living Habits 40%~50% Of Breast Cancer Contain Progesterone Receptor (PR). This Type Of Breast Cancer Is Called PR Positive Breast Cancer. The Commonly Used Breast Cancer Cells In The Experiment Are: MCF-7, MCF-10A, MDA-MB-231, MDA-MB-435, MDA-MB-435S, MDA-MB-436, MDA-MB-453, MDA-MB-468, MDA-MB-157, MDA-MB-361, SK-BR-3, SUM159PT, T-47D, ZR-75-30, ZR-75-1, Hs 578T, HCC1937, HCC 38, HBL100, BT-549, BT-474, BT-20, Bcap-37, DU4475, Etc. The Genes Expressed By Different Cell Lines Will Be Different. The Following Are The Genes Expressed By These Common Cells That We Collate, As Well As The Cell Types Of Breast Cancer That Our Company Can Provide, Teachers In Breast Cancer Research Should Remember To Collect: Cell Name Source/morphological Culture Medium Cell Expression BT-20 Adenocarcinoma Cell Invasive Ductal Carcinoma Breast/epithelial Cell Like Culture Medium: 90% MEM+10% FBS+G/A Complete Culture Medium: MEM Complete Culture Medium (product Number: MD0301) Gas Phase: 37 ℃, 5% CO2ER (-); PR(-); TP53++WT Adherent Growth; The Cells Expressed WNT3 And WNT78. TNF Alpha Inhibited The Cell Growth. This Cell Was Negative For Estrogen Receptor, But Expressed Estrogen MRNA With 5 'exon Deletion. BT-474 Ductal Carcinoma Cell Invasive Ductal Carcinoma Of Breast; Breast/catheter; Epithelial Cell Like Medium: 90% 1640+10% FBS+G/A Complete Medium: 1640 Complete Medium (product No.: MD0201) Gas Phase: 95% Air; 37℃, 5% CO2ER(+); PR(+); HER2(+)； TP53+BT-549 Ductal Carcinoma Cell Breast; Breast/epithelial Cell Culture Medium: 90% 1640+10% FBS+G/A Complete Culture Medium: 1640 Complete Culture Medium (product No.: MD0201) Gas Phase: 95% Air; 37℃, 5% CO2ER(-); PR(-); TP53++WTHBL100  Pleural Effusion Medium: DMEM Basic Medium, 90%; Fetal Bovine Serum, 10%. Gas Phase: 37 ℃, 5% CO2ER (-); PR(-); TP53++HCC1937TNM Stage IIB, Grade 3, Primary Ductal Carcinoma Cell Breast, Breast, Duct; Epithelial Cells, Lymphoblasts Medium: 90% 1640+10% FBS+G/A Complete Medium: 1640 Complete Medium (product No.: MD0201) Gas Phase: 95% Air; 37℃, 5% CO2ER(-); PR(-); TP53-； BRCA1 (mutation, Nucleotide 5382 Inserted Into C), Her2/  Neu-，p53-； Expression Of Epidermal Glycoprotein 2 (EGP2) Cytokeratin 19HCC38TNM Stage IIB, Grade 3, Primary Ductal Carcinoma Cell Breast; Breast/catheter; Epithelial Cell Like Medium: 90% 1640+10% FBS+G/A Complete Medium: 1640 Complete Medium (product No.: MD0201) Gas Phase: 95% Air; 37℃, 5% CO2ER(-); PR(-); TP53++M Adherent Growth; The Cell Oncogene Her2/neu -, P53+; Epithelial Cell Specific Markers EGP2 (epithelial Glycoprotein 2) And CK19 Positive MCF7 Breast Adenocarcinoma Cells Infiltrating Ductal Carcinoma Breast Were Obtained From The Metastasis Site (pleural Effusion)/epithelial Cell Like, Medium: 90% DMEM+10% FBS+G/A Complete Medium: DMEM Complete Medium (product Number: MD0101) Gas Phase: 95% Air; 37℃, 5% CO2ER(+); PR(+); P53 (+/-) WT Expressed WNT7B; Expression Of Insulin Like Growth Factor Binding Protein (IGFBP) BP-2; BP-4; BP-5； TNF- α It Can Inhibit The Growth Of MCF-7 Cells; MCF-10A Breast, Breast; Medium: MEGM Kit (Lonza/Clonics, CC-3150) 100 Ml& Nbsp; The Final Concentration Of Cholera Toxin (Sigma, C8052) Is 100 Ng/ml& Nbsp;& Nbsp;& Nbsp;& Nbsp; Note: MEGM Kit (Lonza/Clonics, CC-3150) Contains 500ml Basic Culture Medium (product No. CC-3151) And 5 Tubes Of Additive Complete Culture Medium: Breast Epithelial Cell Complete Culture Medium (product No. MD1204). Gas Phase: Air 95%; 37℃, 5% CO2ER(-); R(-); TP53 (+/-) WTMDA-MB-157 Medullary Carcinoma Cell Metaplasia Breast, Breast/medulla; Epithelial Cell Like Medium: 90% L-15 (MD1100)+10% Fetal Bovine Serum+G/A Complete Medium: L-15 Complete Medium (product No.: MD1101) Gas Phase: 95% Air; 37℃, 5% CO2ER(-); R(-); TP53(-)。 Wall Growth, Medullary Carcinoma; WNT7B Oncogene Was Expressed. There Were Desmosomes, Microvilli And Tension Filaments At The Boundary Between Cells. MDA-MB-231 Adenocarcinoma Cell Breast/breast; From The Metastatic Site: Pleural Effusion; Epithelial Cell Medium: 90% L-15 (MD1100)+10% Fetal Bovine Serum+G/A Complete Medium: L-15 Complete Medium (product No.: MD1101) Gas Phase: 95% Air; 37℃, 5% CO2< Span Style=" Margin: 0px; Paddi