How To Cultivate Your Cells

Cell Culture Is One Of The Main Tools Of Cell And Molecular Biology, Providing Excellent Model Systems For Studying Normal Cell Physiology And Biochemistry (e.g., Metabolic Research, Aging), Effects Of Drugs And Toxic Compounds On Cells, Mutation And Carcinogenesis. It Is Also Used For Drug Screening And Development, As Well As Large-scale Production Of Biological Compounds (such As Vaccines, Therapeutic Drugs) Proteins. The Main Advantage Of Using Cell Culture In These Applications Is The Consistency And Reproducibility Of The Results Obtained By Using A Batch Of Experiments To Clone Cells& Nbsp; The Culture Medium Is The Most Important Component Of Cell Culture, Because It Provides Necessary Nutrients, Growth Factors And Hormones For Cell Growth, And Regulates The PH Value And Osmotic Pressure Of The Culture. Although The Initial Cell Culture Experiments Were Conducted Using Natural Culture Media Obtained From Tissue Extracts And Body Fluids, The Standardization Of Experiments, The Stable Quality Of Culture Media And The Increased Demand Led To The Development Of Chemically Synthesized Culture Media. At Present, The Synthetic Medium Is Divided Into Basic Medium, Reduced Serum Medium And Serum-free Medium. The Difference Between Them Is That Serum Supplement Is Still Needed. Serum Is An Important Source Of Growth And Adhesion Factors, Hormones And Lipids. And Minerals For Culturing Cells In The Basic Medium. In Addition, Serum Also Regulates The Permeability Of Cell Membrane As The Carrier Of Lipids, Enzymes, Micronutrients, And Trace Elements Into Cells. The Use Of Serum In Culture Medium Has Many Advantages; However, There Are Still Some Unavoidable Disadvantages, Including High Cost, Standardization Problems, Specificity, Variability, Stimulation Or Inhibition Of Growth And/or Cell Function And Other Harmful Effects. In Some Cell Cultures, If The Source Of Serum Is Not Good, It Will Cause Pollution And Pose A Serious Threat To Successful Cell Culture Experiments& Nbsp; Low Serum And Serum-free Culture Have Become One Of The Best Assistants In The Experiment. They Have Greatly Reduced The Cost Of Cell Culture. Low Serum Culture Medium Is One Of The Methods Commonly Used By Many Laboratories To Culture Cells. At Present, We Can Provide ECGM Endothelial Cell Culture Medium Series, Product Number: P1001 (containing 5% Serum), Smooth Muscle Series, Product Number: P3001 (containing 2% Serum), Mesenchymal Stem Cell Series, Product Number: P2001 (containing 5% Serum), Pericyte Series, Article No.: P4001 (containing 5% Serum), Fibroblast Series (P6001), Etc; Its Advantages Are: 1; Reduce The Probability Of Serum In Cell Contamination; 2.  Make The Experiment Repeatable And Consistent 3; Cost Saving. The Low Serum Medium Contains The Protein Needed For Cell Growth. Generally, Conventional Serum Can Support Cells. Disadvantages: 1; Increased Detection Of Cell Culture Medium Proteins; 2.  The Contents And Types Of Protein, Vitamins And Minerals Required For Different Cell Growth Are Different, Which Increases The Difficulty In Preparing Serum Free Culture Media. By Replacing Animal Serum, The Problem Of Using Animal Serum Is Avoided. Protein With Appropriate Nutrition And Hormone Formula Is Included. Many Primary Cultures And Cell Lines Of Serum-free Culture Media Have Formulas. For Example, Our KGM (product Number: P8001, Shanghai Qida Biology) Aims At Keratinocyte Culture. If It Is Used To Feed Epithelial Cells, The Cells Will Be Malnourished. Advantages • More Consistent Performance • Easier Purification And Downstream • Accurate Assessment Of Cell Function • Increased Productivity • Better Control Of Cell Metabolism Disadvantages: • High Cost; • Enhance The Detection Of Cell Culture Medium • Different Cells Need Different Protein, Amino Acid And Vitamin Concentrations And Types. Excessive Concentration Or Incorrect Addition Will Lead To Cell Mutation Or Activation, Increasing The Difficulty Of Preparation& Nbsp;& Nbsp;