Have You Ever Stepped On These Pits Of Frozen Cells?

The Wrong Time To Freeze Cells Mainly Includes: Too Few Cells, Poor Cell Status, Severe Cell Deformation, Or Cell Growth Accumulation. Once The Growth Passes The Flat Top Period, The Culture Medium Will Become Yellow, And The Cells Are Suspected Of Being Polluted; There Are Many Fragments Or Unknown Objects In The Cell Under Microscope; The Culture Time Is Too Long, The Continuous Culture Lasts For More Than Two Months, And The Cell Characteristics Have Changed). Solution: Choose The Best Time For Cell Cryopreservation: The Cell Proliferation Is Vigorous, The Situation Is Stable, The Test Effect Is Good, And Within Two Weeks After Recovery. 1. Too Few Cells: The Cell Concentration Is Lower Than 1-5 During Freezing × 1000,000/ml。 (Recovery Is Hard To Succeed.). Cause: There Will Be Gap Contact Reaction Between Cells. Solution: Adjust The Cell Concentration After Centrifugation, And Put The Cell Seeds Into The Smaller Pore Plate When The Culture Medium Is Very Yellow, The Days After Freezing And Recovery Are Not Long: The Cells Frozen In The Flat Top Period Of Cell Growth Will Stagnate For Several Days After Thawing. The Reason Is That The Cells Have Stopped Growing In The Flat Top Period Of Cell Growth, And The Signals Of Mutual Inhibition Are Maintained After Freezing. The Solution Is To Use Trypsin Digestion To Pave The Board Again; 3. Cell Pollution: The Cover Of The Cryopreservation Tube Must Be Tightened, Otherwise Water Will Seep During The Resuscitation Bath, Causing Pollution. When Resuscitating Cells, Try To Avoid Water At The Joint Of The Cryopreserved Tube. 4. Inactive Cell Resuscitation Caused By The Programmed Cooling Box: Cells Are Being Rapidly Cooled When They Are Placed In The Freezing Box With A Temperature Of - 80 Degrees. The Use Of Ordinary Cryopreservation Solution For Oxidation Resistance And Insufficient Antifreeze Additives Causes A Large Number Of Ice Crystals To Be Generated In The Cells To Kill The Cells. Solution: Use The Finished Cryopreservation Solution Containing Serum (product No.: SD0001) In The Cell Cryopreservation Solution Of Shanghai Qida Exemption Program (product No.: SD0001) Or The Serum Free Cryopreservation Solution (product No.: SD0002), It Contains Antioxidant Factors, Which Can Prevent Cells From Producing Oxidation Reaction When Cells Are Frozen, Giving Cells A Perfect Resting Environment. It Contains Antifreeze Components, Which Can Prevent Most Cells From Being Killed By Ice Crystals. 5 . - The Cell State Is Not Good Because The Temperature Of The Refrigerator Is Difficult To Keep Constant: The Door Is Open/closed, The Voltage Is Unstable, Etc. The Solution Is To Turn Into Liquid Nitrogen As Soon As Possible. 6. Insufficient Liquid Nitrogen: The Liquid Level Cannot Overflow All Cells. Solution: Measure The Liquid Nitrogen Reserve Regularly To Ensure That All Cells Are Immersed Under The Liquid Surface. Small Reminder: Several Cells Need To Be Stored In The Gas Phase And Cannot Be Immersed In Liquid Nitrogen. Pay Attention When Removing Cells. 7. Take The Wrong Cell: Can't Find/take The Wrong Cryotube. Cause: The Cells Were Not Marked And The Inventory Record Was Not Made. Solution: Each Cryopreservation Tube Was Marked With The Name Of The Cells, The Freezing Time, And Recorded.