Important Discovery: CEPT Can Improve The Survival Of Stem Cells

Scientists Have Found A Combination Of Drugs That Can Protect Stem Cells In Culture And Lay A Foundation For Research And Clinical Application& Nbsp; Imagine Working Late On An Important Project. The Next Morning, You Reach Out For A Cup Of Hot Coffee. It's A Kind Of Caffeine Elixir. It Can Cheer You Up, Make Your Thoughts Flow Smoothly, And Prevent You From Being Angry With Your Colleagues& Nbsp; What If There Was A Way To Improve The Performance Of Stem Cells Under Pressure, Just Like A Cup Of Coffee That Can Make People Rejuvenate& Nbsp; Having To Survive In Vitro Is A Huge Burden For Stem Cells. Many People Cannot Survive When They Move From Human Tissues To Cell Culture Bottles, And Those Who Survive Often End Up Weak And Not Functioning Well. When Stem Cells Are Used For Therapeutic Purposes, It Is Particularly Important To Make A Smooth Transition From Healthy And Happy Stem Cells To Safer And More Effective Clinical Outcomes For Patients& Nbsp; Recently, Scientists Have Made Breakthrough Discoveries And Identified A Unique Mixture Of Small Molecules To Support The Health Of Stem Cells Under Laboratory Conditions, Which May Open A New Field In The Application Of These Cells In Regenerative Medicine& Nbsp; In 2006, Shinya Yamanaka, The Nobel Laureate, Discovered Induced Pluripotent Stem Cells (iPSCs), Which Was A Major Turning Point In Research And Medicine. Yamanaka And His Colleagues Cracked The Code Of How To Reverse The Biological Clock Of Adult Cells, And Recombined Their Genes To Make Them Present A Stem Cell Like State. This Discovery Heralds A New Chapter In Regenerative Medicine And Eliminates Many Ethical And Technical Challenges Related To The Use Of Embryonic Stem Cells& Nbsp; Despite This, Although IPSCs Have Performed Well In Drug Discovery And Disease Modeling, We Have Not Yet Fully Exploited Their Potential As Therapeutics. One Of The Problems To Be Solved Is How To Breed And Cultivate Ipsc In A Safe, Controllable And Efficient Way. This Is The First Step To Create A Cell Bank, Which Is A Ready Cell Product Pharmacy That Can Be Deployed To Patients. On The Positive Side, Scientists Have Compiled A List Of How To Distinguish "good" IPSCs From "bad" IPSCs (using Pluripotent Molecular And Cellular Markers). However, Up To Now, There Is Still No Magic Way To Store And Clone IPSCs, And The Tags To Maintain These Viability Are Still Far Away& Nbsp; These Are Expensive And Frustrating Obstacles To The Development Of Cell Therapy. In Clinical Trials Over The Past Few Years, Defective Manufacturing Processes Have Been Identified As The Root Cause Of Poor Stem Cell Performance. These Failures Include Osiris' Prochmal (failure Of Stem Cell Therapy For Transplantation Versus Host Disease) And Brainstorm Cell Therapeutics' Failed Methods For The Treatment Of Amyotrophic Lateral Sclerosis (progressive Disease Of Nervous System)& Nbsp; Stem-cell Espresso Scientists Like Ilyas Singe, Director Of NCATS Stem Cell Translation Laboratory Of The National Institutes Of Health (NIH), Have Been Looking For Ways To Solve These Long-standing Problems Of Stem Cell Culture. Singe ç ç's Laboratory Recently Released A Study Detailing The Groundbreaking Discovery Of A Cocktail Named CEPT, Which Has Been Proven To Significantly Improve IPSC Survival And Eliminate Cell Culture Pressure& Nbsp; "Small Molecule Cocktail Is To Protect Cells And Make The Use Of Stem Cells More Predictable And Effective. In The Process Of Preventing Cell Stress And DNA Damage, We Avoided Cell Death And Improved The Quality Of Living Cells. Singe ç ç Was At The NIH Press Conference." This Cocktail Will Become A Staple Food Widely Used In The Field Of Stem Cells And Promote The Application Of Stem Cells In Research And Clinical. "& Nbsp; Singe ç ç And His Colleagues Used High-throughput Screening Analysis To Discover More Than 15000 Approved Drugs And Research Small Molecules. The Goal Of This Intensive Study Is To Inhibit The Molecules Of ROCK Pathway, Which Is A Cell Signal Transduction Molecule. Once Activated, It Will Reduce The Chance Of Stem Cell Survival. In This Huge Compound Library, The Research Team Found 20 Drugs That Block ROCK, Of Which Chroman 1 Is A Prominent Molecule. Because Chroman 1 Beats The Existing Gold Standard Rock Inhibitor (a Widely Used Compound Y ‑ 27632)& Nbsp; Can Other Molecules Work With Chroman 1 To Improve Cell Survival (think About The Productivity Improvement Of Coffee And Coffee Doughnuts Alone)? The Researchers Used A Matrix Drug Screening System To Search For Successful Combinations And Tested The Effects Of Thousands Of Compounds In A Customized Stem Cell Stress Test& Nbsp; Finally, They Found That A Mixture Consisting Of Chroman 1, Emricasan, Polyamines And Trans ISRIB (or CEPT) Was The Most Effective In Supporting Stem Cell Survival. CEPT Has Been Proved To Greatly Improve Single Cell Cloning, Which Is A Method To Generate Pure Cell Colonies From A Single "cell Seed" IPSC. Single Cell Cloning Is An Advanced Culture Technology, Which Is Used To Improve The Quality And Homogeneity Of Cell Products Entering Patients. However, The Current Culture Is Notoriously Inefficient, And Many Cells Die As A Result Of Forced Reproduction Under Pressure& Nbsp; In Addition, CEPT Also Helped Stem Cells Get Through Another Stressful Experience: Waking Up From Deep Freezing. Stem Cells Used For Research And Clinical Purposes Are Preserved By Cryopreservation, But When They Are Thawed, As Many As 60-70% Of Stem Cells Cannot Survive. Adding CEPT To The Cell Culture Medium Is Very Helpful, Which Increases The Number Of Cells Rebounding From Cold Storage& Nbsp; Singe  ç Colleagues Also Further Studied The Effect Of CEPT Treatment On IPSCs Differentiation And Got More Impressive Results. IPSCs Treated By CEPT Have Formed Healthier And More Powerful Cardiac Cells And Neurons, Which Provides A Possibility For Clinical Treatment& Nbsp; In The Past Two Decades, The Research And Biological Manufacturing Process May Have Come A Long Way, But The Lack Of Clinically Recognized Treatment Methods For IPSC Highlights That This Journey Is Far From Over. If The Breakthrough Methods Of Planting And Harvesting IPSCs, Such As CEPT, Can Be Used, It May Significantly Change The Clinical Pattern In The Next Few Years.