Preparation Of Commonly Used Laboratory Reagents For Dry Goods Sharing

At The Time Of Reagent Preparation, All Kinds Of Algorithms Came. Mathematics And Molecular Principles Were Put Into Action. The Head Was Still Big, But Little Attention Was Paid, And All Previous Efforts Were Wasted. So We Sorted Out Some Conventional Laboratory Preparation Methods For Your Reference: 1mol/L Spermidine: Dissolve 2.55g Of Spermidine In Sufficient Water, So That The Final Volume Is 10ml. Packaged Into Small Portions And Stored At - 20 ℃. 10mol/L Ammonium Acetate: Dissolve 77.1g Of Ammonium Acetate In Water, Add Water To A Constant Volume Of 1L, And Then Filter The Bacteria With A 0.22um Pore Diameter Filter Membrane. 10mg/ml Bovine Serum Albumin (BSA): Add 100mg Of BSA (component V Or Molecular Biology Reagent Grade, No DNA Enzyme) To 9.5ml Of Water (to Reduce Denaturation, Add Protein To Water, Not Water To Protein), Cover It, And Gently Shake It Until The BSA Is Completely Dissolved. Do Not Vortex. Add Water To A Constant Volume Of 10ml, And Then Sub Package Into Small Portions For Storage At - 20 ℃. 1mol/L Dithiothreitol (DTT): Add 32.4ml Water Into The Original Bottle Of 5g Dithiothreitol, And Store It In Small Portions At - 20 ℃. Or Transfer 100mg Dithiothreitol; To A Micro Centrifuge Tube, Add 0.65ml Of Water To Prepare 1mol/L Dithiothreitol Solution. 3mol/L Sodium Acetate: Dissolve 40.8g Of Sodium Acetate Trihydrate In About 90ml Of Water, Adjust The PH Of The Solution To 5.2 With Glacial Acetic Acid, And Then Add Water To Fix The Volume To 100ml. 0.5mol/L EDTA: Prepare Equimolar Na2EDTA And NaOH Solutions (0.5mol/L), And Mix Them To Form Trisodium Salt Of EDTA. Or Weigh 186.1g Of Na2EDTA · 2H2O And 20g Of NaOH, And Dissolve Them In Water To A Constant Volume Of 1L. 1mol/L HEPES: Dissolve 23.8g Of HEPES In About 90ml Of Water, Adjust The PH With NaOH (6.8-8.2), And Then Dilute It To 100ml With Water. 25mg/ml IPGT: Dissolve 250mg Of IPGT (isopropyl Thio- β- D-galactoside) In 10ml Water And Stored In Small Portions At - 20 ℃. 1mol/LMgCl2: Dissolve 20.3g Of MgCl2 · 6H2O In Sufficient Water To A Constant Volume Of 100ml. 100mmol/L PMSF: Dissolve 174mg Of PMSF (phenylmethyl Sulfonyl Fluoride) In Sufficient Isopropyl Alcohol To 10ml. Divide Into Small Portions And Wrap The Filling Tube With Aluminum Foil; Or Stored At - 20 ℃. 20mg/ml Protease K: Add 200mg Protease L Into 9.5ml Water, Gently Shake Until Protease K Is Completely Dissolved. Do Not Vortex. Add Water To A Constant Volume Of 10ml, And Then Sub Package Into Small Portions For Storage At - 20 ℃. 10mg/ml Rnase (DNase Free RNase): Dissolve 10mg Of Trypsin RNA Enzyme In 1ml Of 10mmol/L Sodium Acetate Aqueous Solution (pH 5.0). After Dissolution, It Was Boiled In Water Bath For 15 Min To Inactivate DNase. Adjust The PH To 7.5 With 1mol/L Tris HCl, And Store At - 20 ℃. (Gloves Shall Be Worn During Preparation) 5mol/L Sodium Chloride (NaCl): Dissolve 29.2g Sodium Chloride In Sufficient Water To A Constant Volume Of 100ml. 10N Sodium Hydroxide (NaOH): Dissolve 400g Sodium Hydroxide Particles In A Beaker Of About 0.9L Water (stirred By A Magnetic Stirrer), And Dilute The Volume To 1L With Water After The Sodium Hydroxide Is Completely Dissolved. 2mol/L Sorbitol: Dissolve 36.4g Sorbitol In Sufficient Water To Make The Final Volume 100ml. 100% Trichloroacetic Acid (TCA): Add 100ml Of Water Into The Reagent Bottle Containing 500gTCA, And Stir With A Magnetic Stirrer Until It Is Completely Dissolved. (The Diluent Shall Be Prepared Before Use) 2.5% X-gal (5-bromo-4-chloro-3-indole - β- Galactoside): Dissolve 25mg Of X Gal In 1ml Of Dimethylformamide (DMF), Wrap The Filling Tube With Aluminum Foil, And Store At - 20 ℃& Nbsp; Ca2+and Mg2+free Hanks Solution NaCl 4.5g KCl 0.2g NaHCO3 0.175g Na2HPO4 · 12H2O 0.076g KH2PO4 0.03g Glucose 0.5g 0.4% Phenol Red 2.5ml Dissolve The Above Ingredients In Turn Or Add Them To Double Distilled Water, Finally Add Double Distilled Water To 500ml, Adjust The PH Value To 7.4 With 5.6% NaHCO3, And Store Them In A Refrigerator At 4 ℃ For Standby