Control Of "Gelatina Nigra" In Cell Culture

What Is The "black Glue Insect"/"Nanobacterium"? The First Issue Of The Journal Progress In Biochemistry And Biophysics (PIBB) In 2020 Published An Article Entitled "Detection And Control Of The" Black Glue Insect "pollution In Cell Culture. According To The Author's Description, The "black Glue Insect" Pollution Has The Following Characteristics: 1; There Is No Difference Between Medium And Normal Medium; 2.  At Low Magnification, It Is A Black Dot, Which Is Indistinguishable From The Cell Debris 3; At High Magnification, It Will Exercise For You To See, And It Can Also Imitate The Brownian Motion Of Cell Fragments And Cell Secretions. And The Black Glue Insect "Road Idiot" Runs Everywhere. 4.  It Is Inversely Proportional To The Number Of Cells. The More Cells, The Less They Are, And The More They Are; The Cell State Became Worse With Increasing Nutrition. In Addition To These Conclusions, This Scientific Research Achievement Has Also Successfully Identified The "black Glue Insect" As A New Strain Of Defective Shortwave Monads, Even Named Brevundimonas Sp. GYBL3. Of Course, Some Experimental Results Show That The Initial Infection Of The Black Glue Insect Has No Effect On The Cells And Experiments, But The Subsequent Cell Growth Is Slower And Slower, Or Even Stagnant, With More And More Black Spots, Until The Cells Are "wiped Out" By The Black Glue Insect Control: 1. Try To Use Good Serum, But It Is Generally Expensive, Economic Conditions Do Not Allow, Can Also Choose Imported Newborn Bovine Serum To Replace. It Is Suggested That If Good Serum Is Used, It Is Unnecessary To Inactivate It, So As To Reduce The Formation Of "small Black Spots". 2. If It Is Adherent Cell, It Can Be Washed Several Times With Sterile PBS, Which Can Be Relieved To Some Extent. If It Is Suspended, It Is Not Easy To Deal With It. You Can Add A Few Trophoblasts To It. The Addition Of Trophoblasts Is Very Effective For Newly Revived Cells With Gelatina Melanogaster! If The Cells Recovered For A Long Time, Use PBS To Centrifugate Them For Several Times. The General Recommendation Is 800-1000 Rpm For 3 Minutes, Centrifugate Them For 2-3 Times, Replant Them, And Add Tetracycline And Other Anti Kerria Melanogaster Drugs To Inhibit Them. Let The Cells Grow Up As Soon As Possible, And The Kerria Melanogaster Will Disappear. 3. Change To A Trusted Plastic Culture Bottle. 4. Stop The Resuscitation, Stop The Cell Culture, And Thoroughly Disinfect All The Articles Used For Cell Culture, Including The Culture Bottle, Culture Medium, Straw, Trypsin, Incubator, Ultra Clean Table, Space, Etc. You Can Think Of All The Articles Related To Cell Culture, Of Course, Don't Forget Your White Coat. 5. If There Are Other Available Cells, The Contaminated Cells Should Be Thrown Away; If Not, Try To Kill With Chloramphenicol, Erythromycin, Or Tetracycline. 6. Minocycline Has Inhibitory Effect And Can Be Used When Changing Liquid. As The Black Glue Insect Has Not Been Identified Clearly, The Above Treatment Methods Are Not Necessarily Correct And Can Be Considered For Adoption.