A Simple And Practical Method For Primary Cell Culture

Many Teachers Are Confused When Designing Scientific Research Experiments. What Kind Of Cells Are Good To Use? More And More Teachers Begin To Prefer The Scientific Research Experiments Of Primary Cells. In Designing Experiments, How Should Primary Cells And Cell Lines Be Selected? Definition Of Primary Cells: Primary Cell Culture Refers To The Immediate Culture Of Cells, Tissues And Organs Directly Taken From The Body. Therefore, Strictly Speaking, It Refers To The Culture Before Successful Passage. At This Time, The Cells Retain The Basic Properties Of The Original Cells. If They Are Normal Cells, They Still Retain The Diploid Number. However, In Fact, We Generally Refer To The Cells Cultured From The First Generation To The Tenth Generation As Primary Cell Culture. Because The Primary Cells Can Simulate The Functions Of The Body, They Are Mainly Used In The Biomedical Industry, Such As Drug Screening, Drug Metabolism And Toxicology Research, And Cancer Drug Research. Now We Have Sorted Out Some Tips On Cultivating Primary Cells: Unfreezing Primary Cells Is Very Sensitive To The Unfreezing Process, So It Is Important To Put The Vial In A 37 ℃ Water Bath, Keep It And Gently Rotate It Until The Contents Just Unfreeze. The Vial Should Then Be Immediately Removed From The Water Bath And Transferred To The Sterile Operating Table. Make Sure That Your Culture Bottle Is Ready Before Thawing, So That It Can Be Used To Inoculate Cells Immediately And Placed In The Incubator. When We Use The Primary Retinal Endothelial Cells Of Cell Systems, We Do Not Centrifugate Them When We Resuscitate, But Change The Solution The Next Day. The Primary Cells Of Passage Have Gaps To Inhibit The Adverse Contact Reaction, So The Cells Cannot Be Subcultured When They Reach 100% Density. It Is Generally The Most Effective Suggestion To Observe The Cell Growth Cycle. The Primary Cells Of Cell Systems Are Best Subcultured When The Cell Density Reaches About 85%. When They Grow To 100% Confluence, They Will Age. Remember, All Primary Cells Are Not 100% Pure, So We Should Try To Reduce The Growth Of Contaminated Cells. During Cell Passage, Use Trypsin With Low Concentration, Or Try The Whole Digestion Kit Of Cell Systems (brand: Cell Systems, Product Number: 4Z0-800) And Closely Monitor Cells Under A Microscope. In Addition, Remember To Completely Neutralize Trypsin In Cells After Trypsin Digestion, Because Any Active Trypsin Will Damage Cells. Cryopreservation We Generally Do Not Recommend Refreezing Primary Cells, As This Can Promote Cell Aging And/or Lead To Functional Changes. Primary Cells Are Very Sensitive, And Re Freezing May Lead To Cell Death Or Damage. Unlike Cell Lines, The Proliferation Of Primary Cells Is Limited. We Suggest Using Primary Cells As Early As Possible To Conduct Experiments To Prevent Genetic Drift. If You Are Using A Cell Type With Difficult Proliferation, You Should Closely Monitor The Cell Morphology, Because A Small Number Of Mixed Cells (such As Fibroblasts) May Grow In Large Numbers Over Time And Become The Main Cells& Nbsp; For Normal Primary Cell Culture, It Is Recommended That No Antibody Be Added To The Culture Medium Without Pollution. The Antibody Will Affect Some Gene Variations Of The Cell And Lead To Differences In Experimental Data. Therefore, Cell Systems Will Take This Into Account When Separating And Extracting Cells. They Will Not Use Any Antibody To Separate And Purify Cells At The Same Time, And Make The Cells Reach A Purity Of More Than 95% Through Enzyme Digestion Or Centrifugal Washing, The Experimental Data Obtained In This Way Are More Accurate.