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Is your culture bottle wrapped?
source:QiDa technoligy views:405 time:2024-09-25
When we buy culture bottles, we know that TC-treated culture plates are suitable for adherent cells, because the cells are attached quickly and grow well, while we use non-TC-treated boards for suspended cells, because the surface of the non-TC-treated culture plates is rough, and suspended cells do not need to attach walls, which will grow better.
What about cultured or more difficult cells? There are always people who repeatedly say that the primary cell is less adherent, let's see our solution
Blockbuster recommendation: Cell coated solution (QIDA Bio, No. : SD0044)
Here it comes, here it comes, it is the coating solution of Keida Biology, which is mainly composed of fibronectin, gelatin, and two substrates that promote cell adhesion:
1. It has clear composition and is simple to use. It can be directly added to the culture bottle without preparation and dilution. The inoculated surface of the cells should be completely immersed and then stand still for 4-5 minutes to suck out until the surface of the culture bottle is dry.
2. It contains a variety of components that can not be achieved by fibronectin alone and gelatin solution alone
3. It is suitable for a variety of cells and experiments, and you do not need to prepare a special coating solution for each kind of cell, which can not be put in the refrigerator
4. The key is that it is cheap, only 128 yuan 50ml, and the current price of the activity is 99 yuan 50ml
Are you surprised?
Let's take a look at the effect:
HUVEC extracted with the coated solution, the growth state after 24 hours is as follows:
Control group: HUVEC extracted without coated solution was shown in the following growth state after 24H:
How's that?? It's a little different
Good things need to know how to share acriz, there are already many students have discovered this secret!
That's all for today