UM-Chor1 Mesangial cells

Human mesangial cells UM-Chor1, also known as UM-CHOR-1, UMCHOR1, University of Michigan-Chordoma-1.
Human mesenteric cells UM-Chor1 were isolated in 2012 by Owen JH and colleagues at the University of Michigan from the slope of a 64-day-old white male diagnosed with choroidoma. This cell is the first known human chordoma cell line, exhibiting chordomat-like characteristics with typical chordomoma molecular, genetic, and morphological features. This cell is established from suprasellar lesions with solid, cystic, and hemorrhagic components. It extends along the slope into the sieve sinus. The resulting cultures were purified by flow cytometry, and the cell populations with neuronal adhesion molecule (NCAM) positive staining were expanded into UM-Chor1 cells. Chordoma is a rare and slow-growing tumor type, so UM-Chor1 grows relatively slowly. It has been reported that these cells express chorde-specific transcription factors T (brachyury) and CD34, which can form tumors in NOD/SCID mice. These cells can serve as useful tools for studying the diversity of primary tumors in the spinal cord.

Animal Species:Human

Tissue Source:bone, slope; Disease: Chordia

Morphology:epithelial, grows adherently

Culture Medium Formula:Renal Mesangial Cell Culture Medium(P4301)

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=27102572; DOI=10.1002/path.4729; PMCID=PMC4922416
Susanne Scheipl, Michelle Barnard, Lucia Cottone, Mette Jorgensen, David Harold Drewry, William J. Zuercher, Fabrice Turlais, Hong-Tao Ye, Ana P. Leite, James A. Smith, Andreas Leithner, Peter Moller, Silke Bruderlein, Naomi Guppy, Fernanda Amary, Roberto Tirabosco, Sandra J. Strauss, Nischalan Pillay, Adrienne Margaret Flanagan;
EGFR inhibitors identified as a potential treatment for chordoma in a focused compound screen.
J. Pathol. 239:320-334(2016)

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Daniela Jager, Thomas F.E. Barth, Silke Bruderlein, Angelika Scheuerle, Beate Rinner, Adrian von Witzleben, Andre Lechel, Patrick Meyer, Regine Mayer-Steinacker, Alexandra von Baer, Markus Schultheiss, Christian R. Wirtz, Peter Moller, Kevin Mellert;
HOXA7, HOXA9, and HOXA10 are differentially expressed in clival and sacral chordomas.
Sci. Rep. 7:2032.1-2032.12(2017)

PubMed=28430034; DOI=10.3171/2016.10.JNS16877; PMCID=PMC5650943
John Henry Owen, Christine M. Komarck, Anthony C. Wang, Waleed M. Abuzeid, Richard F. Keep, Erin L. McKean, Stephen Edwin Sullivan, Xing Fan, Mark Edward P. Prince;
UM-Chor1: establishment and characterization of the first validated clival chordoma cell line.
J. Neurosurg. 128:701-709(2018)