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The configuration of Hoechst 33258 dyeing solution is the key. The storage solution is usually at 1mg/mL and needs to be stored in light-proof conditions to prevent the dye from degrading.
Cell-infected bacterial models have extensive applications in many aspects such as pathogens and host interactions, antibacterial drug research and screening, infectious disease treatment research, vaccine effectiveness evaluation and development, environmental monitoring and food safety. However, during the infection process, the phenomenon of mixed bacteria or cell death occurs slightly.