How to extract the little secret of melanocytes

Experimental dogs always have to do the experiment, this is not, just do the week cells, melanocytes and climb the mountains and wade in the rush to come over, pick up surgical scissors, forceps and other experimental small helper began to operate it!

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The operation of cell phagocytosis experiment, the "Hunger games" of cells, was described

Cytophagocytosis is the function of some specific cells in the organism to recognize foreign bodies and swallow and destroy them.

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Master the following 9 tips to easily isolate and culture epidermal keratinocytes

The separation steps of epidermal keratinocytes mainly include the following steps: 1. Take a 1.5mm thick skin sheet and (preferably not more than 12 hours) rinse the obtained skin material with a mixture of 4℃MEM and HBSS for 2-3 times. The ice bath is done in a petri dish. The first time, the blood is flushed away, the second time, the remaining tissue is scraped away, leaving the dermis and epidermis, and the tissue is transferred to a new dish after the first rinse 2. Smooth the skin, cut it into 2-3mm² pieces with a scalpel, and put it into a neutral protease digestion solution for 15-20h at 4℃, or 2-4h at 37℃. 3. Separate dermis and epidermis with sterile needles and ophthalmic tweezers. The epidermis is thin and transparent, and the dermis is thick and slightly swollen and gelatinous under digestion by pancreatic enzymes. 4. The isolated epidermal blocks were digested with 0.25% trypsin and oscillated at 37℃ for 10-30min until the epidermis was organized into flocculent form, and then culture medium containing 10% fetal bovine serum was added to terminate digestion.

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Procedure and precautions for MTT experiment of suspended cells

Procedure and precautions for MTT experiment of suspended cells

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The main cell signaling pathway and query website

Cells are the basic units of living things, but the life activities inside cells are interrelated and interdependent. The formation of these connections and dependencies is inseparable from the signaling pathway between cells. The metabolism of informatio

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Understanding cancer stem cells is critical to the treatment of cancer and the development of new therapies for cancer

Cancer stem cells (CSCs) are a special type of tumor cell that can have a significant impact on the growth and development of debilitating diseases. As the name suggests, cancer stem cells have the ability to differentiate and self-renew, potentially caus

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IPS cell induced differentiation neural cell manipulation

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Continuing from the previous version, other methods for separating blood cells

Continuing from the previous version, other methods for separating blood cells

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Three methods for isolating neutrophils

Three methods for isolating neutrophils

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Worth collecting, steps for staining similar organs

Worth collecting, steps for staining similar organs

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How to extract PBMC cells? Take a look at this sharing

How to extract PBMC cells? Take a look at this sharing

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Briefly describe the basic principles of several commonly used gene mutation detection methods

Briefly describe the basic principles of several commonly used gene mutation detection methods

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XTT method steps for cell activity detection

XTT method steps for cell activity detection

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Do you know these testing methods for angiogenesis?

Do you know these testing methods for angiogenesis?

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Enjoy scientific research and enjoy it freely

Enjoy scientific research and enjoy it freely

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