HCE-T [HCET] Human corneal epithelial cells

The HCE-T cell line was established by transforming the corneal tissue of a 49-year-old woman into SV40 virus, exhibiting typical epithelial cell morphology and capable of producing 64kD keratin. The genetic characteristics of the HCE-T cell line are related to corneal epithelial cells, expressing genes related to corneal epithelial cell function, such as keratin genes. They are widely used as model systems to study various aspects of corneal epithelial biology, including cell adhesion, migration, differentiation, and responses to environmental stimuli and therapeutics. Disease model: dry eye syndrome, corneal infection (bacteria/virus), trauma repair, and corneal neovascularization research. Drug development: Evaluate the permeability and toxicity testing of ophthalmic drugs (such as preservatives benzalkonium chloride) and the delivery effectiveness of nanocarriers. Material testing: biocompatibility analysis of contact lenses and artificial corneal materials. Mechanistic research: inflammatory signaling pathways (NF-κB, MAPK), oxidative stress, and apoptosis mechanisms.

Animal Species: Human 

Tissue Source:Cornea

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:CEpGM Corneal Epithelial Cell Culture Medium（P4101）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=7534282
Kaoru Araki-Sasaki, Yuichi Ohashi, Tetsuo Sasabe, Kozaburo Hayashi, Hitoshi Watanabe, Yasuo Tano, Hiroshi Handa;
An SV40-immortalized human corneal epithelial cell line and its characterization.
Invest. Ophthalmol. Vis. Sci. 36:614-621(1995)

PubMed=20164919; DOI=10.1038/nature08768; PMCID=PMC3145113
Graham Robert Bignell, Christopher D. Greenman, Helen R. Davies, Adam P. Butler, Sarah Edkins, Jenny M. Andrews, Gemma Buck, Lina Chen, David Beare, Calli Latimer, Sara Widaa ...Show all 22 authors... Michael Rudolf Stratton;
Signatures of mutation and selection in the cancer genome.
Nature 463:893-898(2010)

PubMed=26900324; PMCID=PMC4734150
Debra Akin, Jeremy R.B. Newman, Lauren M. McIntyre, Stephen P. Sugrue;
RNA-seq analysis of impact of PNN on gene expression and alternative splicing in corneal epithelial cells.
Mol. Vis. 22:40-60(2016)

PubMed=28315467; DOI=10.1016/j.ejps.2017.03.018
Marina Juretic, Bisera Jurisic Dukovski, Iva Krtalic, Stephan Reichl, Biserka Cetina-Cizmek, Jelena Filipovic-Grcic, Jasmina Lovric, Ivan Pepic;
HCE-T cell-based permeability model: a well-maintained or a highly variable barrier phenotype?
Eur. J. Pharm. Sci. 104:23-30(2017)

DOI=10.22028/D291-35665
Anna-Klara Rubelowski;
Characterization of the HCE-T cell line (RCB 2280) and its comparison with primary limbal epithelial cell culture and primary corneal epithelial cells.
Thesis MD (2018); Saarland University; Saarbrucken; Germany

PubMed=29787296; DOI=10.1080/02713683.2018.1480043; PMCID=PMC6590053
Alison M. McDermott, Hasna Baidouri, Ashley M. Woodward, Wendy R. Kam, Yang Liu, Xiao-Min Chen, Jillian F. Ziemanski, Kerry Vistisen, Linda D. Hazlett, Kelly K. Nichols, Pablo Argueso, David A. Sullivan;
Short tandem repeat (STR) profiles of commonly used human ocular surface cell lines.
Curr. Eye Res. 43:1097-1101(2018)

PubMed=31927639; DOI=10.1007/s00417-019-04563-0
Anna-Klara Rubelowski, Lorenz Latta, Priya Katiyar, Tanja Stachon, Barbara Kasmann-Kellner, Berthold Seitz, Nora Szentmary;
HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium.
Graefes Arch. Clin. Exp. Ophthalmol. 258:565-575(2020)