CHONDROGENIC differentiation Culture medium. Chondrogenic differentiation culture medium

Product Description

Mesenchymal stem cell cartilage differentiation kit is a self-developed low-serum medium for human mesenchymal stem cells to induce cartilage differentiation. Using this medium to culture cells can be used to induce cartilage differentiation of mesenchymal stem cells from bone marrow, umbilical cord, fat and other tissues.

Medium formula:

Basic medium:500ML, cell growth factor 5ml, chondroblast-inducible factor 5ml, fetal bovine serum: 25ml (SD0200), G/A: 5ml (SD0038)

• The procedure of inducing differentiation is simple and convenient.

• The induction efficiency of chondrocytes is high.

   Mesenchymal stem cell cartilage induction (Day12)

Induction steps:

One、 The cell culture plate is coated with cell cell coating solution, and the stem cells that have been passed or revived and frozen are inoculated on the 6-well plate with a density of 2 × 105 -3 × 105 cells/cm2 or 2 × 105 -3 × 105 cells/well, cultured in a 37 ℃, 5% CO2 incubator. When using cell growth factor to reach 80% - 90% of the cell confluence, carefully suck out the cell culture medium in the well, and add 2mL of cell cartilage differentiation culture medium to the 6-well plate, which is recorded as the 0 th day;

Note: Polylysine solution (article number: SD0042) is required for cell coating.

Two、 Replace fresh cell cartilage differentiation medium every 1-2 days;

Note: In order to prevent the chondrocyte from falling off, it is suggested that after a large number of calcium nodules appear in the induction process, the form of fluid change should be changed to one and a half times a day.

Three、 During 14-21 days of induction, the cells were stained according to their morphological changes and growth.

Four、 After the induction of osteogenic differentiation, the cartilage differentiation medium in the 6-well plate was sucked and washed with PBS for 1-2 times. Add 1 mL of cell fixation solution into each well and fix for 10~30 minutes;

Five、 Suck off the cell fixative and wash it with PBS twice. Add 2 mL of cartilage detection dye solution to each well for 3-5 min;

Six、 Suck off the dye solution for cartilage detection and wash it with PBS for 2-3 times;

Seven、 Place the culture plate under the microscope to observe the staining effect

Statement: The product is for scientific research only.