HCCLM3 Human Liver Cancer Cells (high Conversion)

The cell was sampled from a 39-year-old male patient and passed STR/non-mycoplasma detection in this library. Nude mice were inoculated with human hepatoma cell line MHCC97-H for three times of lung metastasis screening, and the lung metastatic tumor was selected to build a hepatoma cell line with highly spontaneous lung metastasis after subcutaneous inoculation.

Animal species/Organism human

Gender Male

Tissue and Cell Type liver cancer

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=12133480

Li Y., Tang Z.-Y., Ye S.-L., Liu Y.-K., Chen J., Xue Q., Huang X.-W., Chen J., Bao W.-H., Yang J., Gao D.-M.

Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection.

Zhonghua Yi Xue Za Zhi 82:601-605(2002)

PubMed=12618900; DOI=10.1007/s00432-002-0396-4

Li Y., Tang Y., Ye L., Liu B., Liu K., Chen J., Xue Q.

Establishment of a hepatocellular carcinoma cell line with unique metastatic characteristics through in vivo selection and screening for metastasis-related genes through cDNA microarray.

J. Cancer Res. Clin. Oncol. 129:43-51(2003)

PubMed=15796966; DOI=10.1016/j.cancergencyto.2004.05.010

Yang J., Qin L.-X., Li Y., Ye S.-L., Liu Y.-K., Gao D.-M., Chen J., Tang Z.-Y.

Molecular cytogenetic characteristics of the human hepatocellular carcinoma cell line HCCLM3 with high metastatic potential: comparative genomic hybridization and multiplex fluorescence in situ hybridization.

Cancer Genet. Cytogenet. 158:180-183(2005)

PubMed=19335983

Zheng D., Liu B.-B., Liu Y.-K., Kang X.-N., Sun L., Guo K., Sun R.-X., Chen J., Zhao Y.

Analysis of the expression of Slit/Robo genes and the methylation status of their promoters in the hepatocellular carcinoma cell lines.

Zhonghua Gan Zang Bing Za Zhi 17:198-202(2009)