Hippocampal Neurons Of HT-22 Mice

HT-22 is an immortal mouse hippocampal cell line (1) subcloned from HT-4 cell line. The parent HT-4 cell line is derived from immortalization of mouse neuron tissue with temperature sensitive SV40 T antigen (2). HT-22 is highly sensitive to glutamate, so it is often used as a model system to study the neurotoxicity induced by glutamate.

Animal species/Organism mice

Tissue and Cell Type Brain; a hippocampus; neuron

Morphology nerve cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

1.   Wu, S.; Huang, R.; Zhang, R.; Xiao, C.; Wang, L.; Luo, M.; Song, N.; Zhang, J.; Yang, F.; Liu, X.; et al. Gastrodin and Gastrodigenin Improve Energy Metabolism Disorders and Mitochondrial Dysfunction to Antagonize Vascular Dementia. Molecules 2023, 28, 2598. 

    https://doi.org/10.3390/molecules28062598