K7M2 Wt Mouse Osteosarcoma Osteoblasts

Antigen expression: CD31 positive [PuMed: 1315100] Product: eight factors [PuMed: 11315100]; Integrated sialic acid protein (BSP) [PuMed11315100]; biglyan [PubMed: 11315100]; decorrin [PubMed:1315100]; villin 2 (ezrin) [PubMed: 11325848]. The expression of bone sialoprotein, biglyan, decorin, and osteopontin showed the characteristics of bone family cells. It was put into storage after identification without mycoplasma.

Tissue and Cell Type

Strain: BALB/c

Organ: bone

Disease: osteosarcoma

Source metastatic focus: lung

Cell type: osteoblast;

Morphology osteoblast-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (article number: MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=11315100; DOI=10.1023/A:1006767007547

Khanna C., Prehn J., Yeung C., Caylor J., Tsokos M., Helman L.

An orthotopic model of murine osteosarcoma with clonally related variants differing in pulmonary metastatic potential.

Clin. Exp. Metastasis 18:261-271(2000)

PubMed=17671136; DOI=10.1158/1078-0432.CCR-07-0313

Gordon N., Koshkina N.V., Jia S.-F., Khanna C., Mendoza A., Worth L.L., Kleinerman E.S.

Corruption of the Fas pathway delays the pulmonary clearance of murine osteosarcoma cells, enhances their metastatic potential, and reduces the effect of aerosol gemcitabine.

Clin. Cancer Res. 13:4503-4510(2007)

PubMed=26320182; DOI=10.18632/oncotarget.5177

Ren L., Mendoza A., Zhu J., Briggs J.W., Halsey C., Hong E.S., Burkett S.S., Morrow J., Lizardo M.M., Osborne T., Li S.Q., Luu H.H., Meltzer P.S., Khanna C.

Characterization of the metastatic phenotype of a panel of established osteosarcoma cells.

Oncotarget 6:29469-29481(2015)