Ins-1 Rat Insulinoma Cells

This cell is derived from rats transplanted with islet tumor irradiated by X-rays. It is insulin positive and can synthesize proinsulin I and II, which can be used for the study of beta cell function. Through mycoplasma detection in this library.

Tissue and Cell Type: Islet cells

Morphology:Irregular type； adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

RPMI 1640 (MD0200)+10% fetal bovine serum (SD0200)+50 μ Mol/L 2-mercaptoethanol (SD0031)+1% GA (SD0038)

Link to complete medium: RPMI 1640 complete medium (MD0206)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=1370150; DOI=10.1210/endo.130.1.1370150

Asfari M., Janjic D., Meda P., Li G., Halban P.A., Wollheim C.B.

Establishment of 2-mercaptoethanol-dependent differentiated insulin-secreting cell lines.

Endocrinology 130:167-178(1992)

PubMed=22538498; DOI=10.1159/000338793

Grozinsky-Glasberg S., Shimon I., Rubinfeld H.

The role of cell lines in the study of neuroendocrine tumors.

Neuroendocrinology 96:173-187(2012)