Rat small intestinal fibroblasts-

Rat Intestinal Fibroblasts from AcceGen are isolated from small intestinal tissue of 1-day-old neonatal Sprague-Dawley rats. Rat Primary Intestinal Fibroblasts are grown in T75 tissue culture flasks pre-coated with gelatin-based coating solution for 2 min and incubated in AcceGen Culture Complete Growth Medium generally for 2-5 days. Cultures are then expanded. Prior to shipping, cells are detached from flasks and immediately cryo-preserved in vials. Each vial contains at least 1x106 cells per ml and is delivered frozen.

Medium Formulation:

90% DMEM (MD0100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

DMEM complete medium (MD0101)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only