HARA-B Human Lung Squamous Cell Carcinoma Cells

Initially human cells, but the cell line was established from the metastatic organ (bone) of nude mice. The cell line highly expressed PTHrP, and intracardiac injection of nude mice can widely induce bone metastasis. Subcutaneous transplantation of cells in mice can cause hypercalcemia.

Animal species/Organism Human

Tissue and Cell Type Lung, Squamous Cell Carcinoma

Morphology epithelial cell-like, adherent growth

Medium Formulation:

90% 1640 (MD0200)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: 1640 complete medium (MD0201)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

PubMed=8752176

Iguchi H., Tanaka S., Ozawa Y., Kashiwakuma T., Kimura T., Hiraga T., Ozawa H., Kono A.

An experimental model of bone metastasis by human lung cancer cells: the role of parathyroid hormone-related protein in bone metastasis.

Cancer Res. 56:4040-4043(1996)

PubMed=11668474; DOI=10.1002/ijc.1425

Iguchi H., Onuma E., Sato K., Sato K., Ogata E.

Involvement of parathyroid hormone-related protein in experimental cachexia induced by a human lung cancer-derived cell line established from a bone metastasis specimen.

Int. J. Cancer 94:24-27(2001)