CRFK Cat Renal Epithelial Cells

The cell line was established by RAVan Deusen. After detection, the cell line was not infected with bovine viral diarrhea virus. It was put into storage after identification without mycoplasma.

Animal species/Organism cat

Tissue and Cell Type kidney, epithelial cells

Morphology epithelial cell-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (article number: MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)

Note: This product is for scientific research only

PubMed=4130570; DOI=10.1007/BF02618435

Crandell R.A., Fabricant C.G., Nelson-Rees W.A.

Development, characterization, and viral susceptibility of a feline (Felis catus) renal cell line (CRFK).

In Vitro 9:176-185(1973)

PubMed=9696876

Baumann J.G., Gunzburg W.H., Salmons B.

CrFK feline kidney cells produce an RD114-like endogenous virus that can package murine leukemia virus-based vectors.

J. Virol. 72:7685-7687(1998)

PubMed=20631117; DOI=10.1128/JCM.00992-10

Yoshikawa R., Sato E., Igarashi T., Miyazawa T.

Characterization of RD-114 virus isolated from a commercial canine vaccine manufactured using CRFK cells.

J. Clin. Microbiol. 48:3366-3369(2010)

PubMed=21029758; DOI=10.1016/j.virusres.2010.10.020

Okada M., Yoshikawa R., Shojima T., Baba K., Miyazawa T.

Susceptibility and production of a feline endogenous retrovirus (RD-114 virus) in various feline cell lines.

Virus Res. 155:268-273(2011)

PubMed=23585909; DOI=10.1371/journal.pone.0061530

Fukuma A., Yoshikawa R., Miyazawa T., Yasuda J.

A new approach to establish a cell line with reduced risk of endogenous retroviruses.

PLoS ONE 8:E61530-E61530(2013)