C6/36 Aedes Albopictus Cells

The C6/36 cell line was established in 1978 (Igarashi. A), and then cloned several times. The cell growth state is stable and can be successfully used for the proliferation of a variety of viruses. It was put into storage after identification without mycoplasma.

Animal species/Organism RIII larvae

Tissue and Cell Type juvenile mosquito cells

Morphology epithelial cell-like, adherent growth

Medium Formulation:

90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: MEM complete medium (MD0301)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 33.5 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution:No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

PubMed=690610; DOI=10.1099/0022-1317-40-3-531

Igarashi A.

Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.

J. Gen. Virol. 40:531-544(1978)

CLPUB00466

Herrera R.J.

Cytological, cytogenetic and biochemical studies on several permanent insect cell lines.

Thesis PhD (1982), Fordham University, United States

PubMed=6126429; DOI=10.1007/BF02796333

Brown S.E., Knudson D.L.

Characterization of invertebrate cell lines. IV. Isozyme analyses of dipteran and acarine cell lines.

In Vitro 18:347-350(1982)

PubMed=6137452; DOI=10.1007/BF02628962

Kuno G.

Cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication.

In Vitro 19:707-713(1983)

PubMed=2861916

Kuno G., Gubler D.J., Velez M., Oliver A.

Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses.

Bull. World Health Organ. 63:279-286(1985)

PubMed=3611315

White L.A.

Susceptibility of Aedes albopictus C6/36 cells to viral infection.

J. Clin. Microbiol. 25:1221-1224(1987)

PubMed=2563994; DOI=10.1007/BF02626177

Kuno G., Oliver A.

Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses.

In Vitro Cell. Dev. Biol. 25:193-196(1989)

PubMed=19951376; DOI=10.1111/j.1365-2672.2009.04621.x

Voronin D., Tran-Van V., Potier P., Mavingui P.

Transinfection and growth discrepancy of Drosophila Wolbachia strain wMel in cell lines of the mosquito Aedes albopictus.

J. Appl. Microbiol. 108:2133-2141(2010)

PubMed=20976219; DOI=10.1371/journal.pone.0013398

Frentiu F.D., Robinson J., Young P.R., McGraw E.A., O'Neill S.L.

Wolbachia-mediated resistance to dengue virus infection and death at the cellular level.

PLoS ONE 5:E13398-E13398(2010)

PubMed=28404849; DOI=10.1128/JVI.00554-17

Xin Q.-L., Deng C.-L., Chen X., Wang J., Wang S.-B., Wang W., Deng F., Zhang B., Xiao G.-F., Zhang L.-K.

Quantitative proteomic analysis of mosquito C6/36 cells reveals host proteins involved in Zika virus infection.

J. Virol. 91:e00554.17-e00554.17(2017)

PubMed=29329394; DOI=10.1093/gigascience/gix135

Miller J.R., Koren S., Dilley K.A., Puri V., Brown D.M., Harkins D.M., Thibaud-Nissen F., Rosen B., Chen X.-G., Tu Z., Sharakhov I.V., Sharakhova M.V., Sebra R., Stockwell T.B., Bergman N.H., Sutton G.G., Phillippy A.M., Piermarini P.M., Shabman R.S.

Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation.

GigaScience 7:gix135-gix135(2018)

PubMed=29973702; DOI=10.1038/s41598-018-28357-2

Gaburro J., Duchemin J.-B., Paradkar P.N., Nahavandi S., Bhatti A.

Electrophysiological evidence of RML12 mosquito cell line towards neuronal differentiation by 20-hydroxyecdysdone.

Sci. Rep. 8:10109-10109(2018)