NCI-H889 [H889] Small cell lung cancer cells

Human small cell lung cancer cells NCI-H889 [H889], also known as H-889 or NCIH889, are epithelial-shaped cell lines isolated by Gazdar AF and colleagues from the lung tissue of a 69-year-old white woman diagnosed with lung cancer. Compared to normal lung tissue, these cells expressed low levels of p53 mRNA (a mutation at base 725 of p53 with G→C causing the amino acid Cys →Ser at position 242) and a K-ras 12 mutation. These cells can be used for cancer research and three-dimensional cell culture.

Animal Species: Human

Tissue Source:Lungs, from metastatic sites: lymph nodes; Disease: Small cell lung cancer, stage E

Morphology:Epithelial, suspended growth, clustered or clustered

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

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