LN-18 Human brain glioblastoma cells

Human brain glioblastoma cell LN-18, also known as LN-18, LN18, LN018.
LN-18 cells were isolated in 1976 from the right temporal lobe of a 65-year-old white male with glioblastoma, exhibiting epithelial morphology. Reportedly, the cells express the Fibronectin gene but do not express GFAP (glial fibrin acidic protein) and S100 protein. The tumor suppressor genes p16 and p14ARF are deletion in this cell, TP53 mutation (amino acid Cys at position 238 is mutated to Ser), but the PTEN gene is normal. Fas ligand stimulates cells for 16 hours, leading to apoptosis; Purinomycin's cytotoxic effect is dose-dependent. Bcl-2 protects these cells from Fas ligand-induced cell death, but has only a limited protective effect against purinemycin-induced apoptosis. Additionally, these cells are tumorigenic and can form tumors in nude mice.

Animal Species:Human

Tissue Source:Brain, right temporal lobe; Disease: glioblastoma (Kernohan Grade IV)

Morphology:epithelial, grows adherently

Culture Medium Formula:DMEM (high Sugar) Complete Medium(MD0103)

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

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