AMC-HN-8 Human laryngeal cancer cells

AMC-HN-8 was established in 1996 by the Asan Medical Center from carcinoma in situ or metastatic sites of nine head and neck cancer patients, establishing nine head and neck cancer cell lines. By transplanting fresh tumor tissue, proliferation and development of immortal tumor cell lines. AMC-HN-8 grows as a wall-adherent epithelium-like monolayer and is tested for tumorigenization and tissue testing in nude mice. Flow cytometry and indirect immunofluorescence were used to analyze DNA content and the expression of integrin subunits α6, β4, β1, and intracellular binder 1 (ICAM-1). This strain of cells originates from squamous cell carcinoma. β4 is highly expressed. AMC-HN-8 cells are a representative in vitro model for head and neck tumor biology research.

Animal Species: Human

Tissue Source:Head and neck squamous cell carcinoma

Morphology:Epithelial cells, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=9349880; DOI=10.3109/00016489709113477
Sang Yoon Kim, Kwang-Chol Chu, Hye Rim Lee, Kwang-Sun Lee, Thomas E. Carey;
Establishment and characterization of nine new head and neck cancer cell lines.
Acta Oto-Laryngol. 117:775-784(1997)

PubMed=24336076; DOI=10.1038/cddis.2013.488; PMCID=PMC3877559
Jong-Lyel Roh, Eun Hye Kim, H.B. Park, Jin Young Park;
The Hsp90 inhibitor 17-(allylamino)-17-demethoxygeldanamycin increases cisplatin antitumor activity by inducing p53-mediated apoptosis in head and neck cancer.
Cell Death Dis. 4:e956.1-e956.8(2013)