RIN-m5F rat pancreatic islet β-cell tumor cells

RIN-m5F cells are clones of rat pancreatic islet cells RIN-m, which secrete insulin and L-dopa decarboxylase but do not produce growth hormone-inhibiting hormone.

Animal Species: Rat 

Tissue Source: Islet cell tumor

Morphology: Epithelioid in appearance, adherent growth

Culture Medium Formula:RPMI-1640 Complete Medium（MD0201）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=6295859; DOI=10.2337/diab.31.6.521
Sam J. Bhathena, Herbert Kazuto Oie, Adi F. Gazdar, Nancy R. Voyles, Samuel D. Wilkins, Lillian Recant;
Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor.
Diabetes 31:521-531(1982)

PubMed=6129963; DOI=10.1210/endo-112-3-1070
Herbert Kazuto Oie, Adi F. Gazdar, John D. Minna, Gordon C. Weir, Stephen Bruce Baylin;
Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor.
Endocrinology 112:1070-1075(1983)

PubMed=6141566; DOI=10.3181/00379727-175-41762
Sam J. Bhathena, Sissay Awoke, Nancy R. Voyles, Samuel D. Wilkins, Lillian Recant, Herbert Kazuto Oie, Adi F. Gazdar;
Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones.
Proc. Soc. Exp. Biol. Med. 175:35-38(1984)

PubMed=2997172; DOI=10.1016/S0021-9258(17)38764-1
David Andrew Nielsen, Michael Welsh, Malcolm John Casadaban, Donald Frederick Steiner;
Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. I. Effects of glucose and cyclic AMP on the transcription of insulin mRNA.
J. Biol. Chem. 260:13585-13589(1985)

PubMed=3902821; DOI=10.1016/S0021-9258(17)38765-3
Michael Welsh, David Andrew Nielsen, Albert James MacKrell Jr., Donald Frederick Steiner;
Control of insulin gene expression in pancreatic beta-cells and in an insulin-producing cell line, RIN-5F cells. II. Regulation of insulin mRNA stability.
J. Biol. Chem. 260:13590-13594(1985)

PubMed=2550270; DOI=10.1055/s-0029-1210869
Jens Hoiriis Nielsen;
Mechanisms of pancreatic beta-cell growth and regeneration: studies on rat insulinoma cells.
Exp. Clin. Endocrinol. 93:277-285(1989)

PubMed=2569024; DOI=10.1677/joe.0.1210479
David George Lambert, Terence William Atkins;
Effects of islet hormones on insulin secretion from cloned B cell lines, HIT-T15 and RINm5F.
J. Endocrinol. 121:479-485(1989)

PubMed=2090331; DOI=10.1016/0922-3371(90)90097-G
Raphael K. Bartholomeusz, Iain L. Campbell, Leonard Charles Harrison;
A2B5-reactive ganglioside expression determines the differentiation stage and capacity of rat insulinoma (RIN) sublines.
Cell Differ. Dev. 32:39-46(1990)

PubMed=2156672; DOI=10.1210/endo-126-4-1927
Raphael K. Bartholomeusz, Iain L. Campbell, Leonard Charles Harrison;
A2B5-reactive ganglioside expression is an index of differentiation in rat insulinoma (RIN) cells.
Endocrinology 126:1927-1933(1990)

PubMed=8697299
Vincent Poitout, Lawrence Karl Olson, R. Paul Robertson;
Insulin-secreting cell lines: classification, characteristics and potential applications.
Diabetes Metab. 22:7-14(1996)

PubMed=10417352; DOI=10.1042/bj3410847; PMCID=PMC1220426
Carolyn Ewen Fiskerstrand, Paul Newey, Bahram Ebrahimi, Lesley Gerrard, Patrick Harrison, Gerard P. McGregor, John P. Quinn;
Novel cell lines for the analysis of preprotachykinin A gene expression identify a repressor domain 3' of the major transcriptional start site.
Biochem. J. 341:847-852(1999)

DOI=10.1016/j.bej.2003.09.013
Akiko Ogawa, Satoshi Terada, Yasuyuki Saito, Toshihisa Kimura, Akio Yamaguchi, Masao Miki;
CrmA and Bcl-2 protect a cell line derived from islet of Langerhans, RIN-5F from hypoxia-induced apoptosis.
Biochem. Eng. J. 20:209-215(2004)

PubMed=22538498; DOI=10.1159/000338793
Simona Grozinsky-Glasberg, Ilan Shimon, Hadara Rubinfeld;
The role of cell lines in the study of neuroendocrine tumors.
Neuroendocrinology 96:173-187(2012)