C6/36 juvenile mosquito cells

The C6/36 larval cell line, also known as Aedes albopictus clone C6/36, Clone C6/36, or C6-36, is a spontaneously immortalized cell line isolated from larvae of Aedes albopictus (Asian tiger mosquito). It has been reported to be suitable for flavivirus replication and high-titer propagation of dengue virus. C6/36 serves as an appropriate transfection host with a diploid nuclear karyotype. The cells exhibit anchorage-independent growth, form colonies in semi-solid culture media, but do not develop tumors in immunosuppressed mice.

Animal Species: Larva 

Tissue Source: Larva

Morphology: Adherent growth, epithelial

Culture Medium Formula:MEM Complete Medium（MD0301）

Gas phase: Air, 95%; Carbon dioxide, 5%. Temperature: 37℃. 

Freezing conditions:  90% FBS (SD0200) + 10% DMSO

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: The product is for scientific research purposes only.

PubMed=690610; DOI=10.1099/0022-1317-40-3-531
Akira Igarashi;
Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses.
J. Gen. Virol. 40:531-544(1978)

CLPUB00466
Rene J. Herrera;
Cytological, cytogenetic and biochemical studies on several permanent insect cell lines.
Thesis PhD (1982); Fordham University; New York; USA

PubMed=6126429; DOI=10.1007/BF02796333
Susan E. Brown, Dennis L. Knudson;
Characterization of invertebrate cell lines. IV. Isozyme analyses of dipteran and acarine cell lines.
In Vitro 18:347-350(1982)

PubMed=6137452; DOI=10.1007/BF02628962
Goro Kuno;
Cultivation of mosquito cell lines in serum-free media and their effects on dengue virus replication.
In Vitro 19:707-713(1983)

PubMed=2861916; PMCID=PMC2536393
Goro Kuno, Duane J. Gubler, Miriam Velez, Antonio Oliver;
Comparative sensitivity of three mosquito cell lines for isolation of dengue viruses.
Bull. World Health Organ. 63:279-286(1985)

PubMed=3951024; DOI=10.1128/JVI.58.1.81-86.1986; PMCID=PMC252879
Lynn D. Condreay, Dennis T. Brown;
Exclusion of superinfecting homologous virus by Sindbis virus-infected Aedes albopictus (mosquito) cells.
J. Virol. 58:81-86(1986)

PubMed=3611315; DOI=10.1128/jcm.25.7.1221-1224.1987; PMCID=PMC269180
Lendell A. White;
Susceptibility of Aedes albopictus C6/36 cells to viral infection.
J. Clin. Microbiol. 25:1221-1224(1987)

PubMed=3392770; DOI=10.1128/JVI.62.8.2629-2635.1988; PMCID=PMC253693
Lynn D. Condreay, Raquel H. Adams, Judy Edwards, Dennis T. Brown;
Effect of actinomycin D and cycloheximide on replication of Sindbis virus in Aedes albopictus (mosquito) cells.
J. Virol. 62:2629-2635(1988)

PubMed=2563994; DOI=10.1007/BF02626177
Goro Kuno, Antonio Oliver;
Maintaining mosquito cell lines at high temperatures: effects on the replication of flaviviruses.
In Vitro Cell. Dev. Biol. 25:193-196(1989)

PubMed=19951376; DOI=10.1111/j.1365-2672.2009.04621.x
Denis A. Voronin, Van Tran-Van, Patrick Potier, Patrick Mavingui;
Transinfection and growth discrepancy of Drosophila Wolbachia strain wMel in cell lines of the mosquito Aedes albopictus.
J. Appl. Microbiol. 108:2133-2141(2010)

PubMed=20976219; DOI=10.1371/journal.pone.0013398; PMCID=PMC2955527
Francesca D. Frentiu, Jodie Robinson, Paul R. Young, Elizabeth Ann McGraw, Scott L. O'Neill;
Wolbachia-mediated resistance to dengue virus infection and death at the cellular level.
PLoS ONE 5:e13398.1-e13398.8(2010)

PubMed=28404849; DOI=10.1128/JVI.00554-17; PMCID=PMC5446628
Qi-Lin Xin, Cheng-Lin Deng, Xi Chen, Jun Wang, Shao-Bo Wang, Wei Wang, Fei Deng, Bo Zhang, Geng-Fu Xiao, Lei-Ke Zhang;
Quantitative proteomic analysis of mosquito C6/36 cells reveals host proteins involved in Zika virus infection.
J. Virol. 91:e00554.17.1-e00554.17.17(2017)

PubMed=29329394; DOI=10.1093/gigascience/gix135; PMCID=PMC5869287
Jason R. Miller, Sergey Koren, Kari A. Dilley, Vinita Puri, David M. Brown, Derek M. Harkins, Francoise Thibaud-Nissen, Benjamin Rosen, Xiao-Guang Chen, Zhi-Jian Tu, Igor V. Sharakhov, Maria V. Sharakhova, Robert P. Sebra, Timothy B. Stockwell, Nicholas H. Bergman, Granger G. Sutton, Adam M. Phillippy, Peter M. Piermarini, Reed S. Shabman;
Analysis of the Aedes albopictus C6/36 genome provides insight into cell line utility for viral propagation.
GigaScience 7:gix135.1-gix135.13(2018)

PubMed=29973702; DOI=10.1038/s41598-018-28357-2; PMCID=PMC6031678
Julie Gaburro, Jean-Bernard Duchemin, Prasad N. Paradkar, Saeid Nahavandi, Asim Bhatti;
Electrophysiological evidence of RML12 mosquito cell line towards neuronal differentiation by 20-hydroxyecdysdone.
Sci. Rep. 8:10109.1-10109.11(2018)

PubMed=33389257; DOI=10.1007/s10096-020-04106-0; PMCID=PMC7778494
Nathalie Wurtz, Gwilherm Penant, Priscilla Jardot, Nathalie Duclos, Bernard La Scola;
Culture of SARS-CoV-2 in a panel of laboratory cell lines, permissivity, and differences in growth profile.
Eur. J. Clin. Microbiol. Infect. Dis. 40:477-484(2021)