HK-1 Nasopharyngeal Carcinoma Cell Line

HK-1 cells (full name: NPC/HK-1) are a human nasopharyngeal squamous carcinoma cell line established in 1979-1980 by Huang et al. at the University of Hong Kong, derived from the nasopharyngeal carcinoma tissues of a 58-year-old male patient who had recurred after 17 and a half years of radiotherapy, and are one of the most widely used in vitro models of nasopharyngeal carcinoma internationally.

Organisational source: Recurrent highly differentiated squamous carcinoma of the nasopharynx.

Morphological:Epithelioid, monolayer adherent growth.

Medium Formulation:

90%RPMI-1640(MD0200)+10%FBS(SD0200)+G/A (SD0038)

Complete Medium：RPMI-1640（MD0201）

Gaseous phase: air, 95 %; carbon dioxide, 5 %. Temperature: 37°C

Complete freezing solution: No Programmed Cryopreservation Cells Solution (low Serum)SD0001

Declaration: This product is for scientific research only.

1.PubMed=6259064; DOI=10.1002/ijc.2910260202
Dolly Wai-Sen Huang Poon, John Hung-Chiu Ho, Yeuk-Foo Poon, Eng-Ching Chew, Daisy Saw, M. Lui, C.L. Li, L.S. Mak, S.H. Lai, Wai-Hon Lau;
Establishment of a cell line (NPC/HK1) from a differentiated squamous carcinoma of the nasopharynx.
Int. J. Cancer 26:127-132(1980)

2.PubMed=12645650; DOI=10.1016/S0165-4608(02)00657-X
Nathalie Wong, Angela Bik-Yu Hui, Bo-Li Fan, Kwok-Wai Lo, Elizabeth Pang, Sing-Fai Leung, Dolly Wai-Sen Huang Poon, Philip James Johnson;
Molecular cytogenetic characterization of nasopharyngeal carcinoma cell lines and xenografts by comparative genomic hybridization and spectral karyotyping.
Cancer Genet. Cytogenet. 140:124-132(2003)

3.PubMed=18196576; DOI=10.1002/ijc.23374
Sylvia Yat-Yee Chan, Kwong-Wai Choy, George Sai-Wah Tsao, Qian Tao, Tao Tang, Grace Tin-Yun Chung, Kwok-Wai Lo;
Authentication of nasopharyngeal carcinoma tumor lines.
Int. J. Cancer 122:2169-2171(2008)

4.PubMed=39905741; DOI=10.1002/cam4.70422; PMCID=PMC11794828
Anna Makowska, H. Udo Kontny, Josef van Helden, Barbara Hildebrandt, Herdit M. Schuler, Ralf Weiskirchen;
Genetic characterization of the immortalized human nasopharyngeal carcinoma cell line NPC/HK1.
Cancer Med. 14:e70422.1-e70422.8(2025)