Tumor stem cell pellet culture medium - no serum

The sphere culture does not need a scaffold, and forms a cell sphere through the characteristics of adherent cells tending to gather, which is one of the most common and universal non-scaffold methods used for 3D cell culture. The commonly used spheres include embryonic bodies, breast spheres, tumor spheres, hepatocyte spheres and nerve spheres, which are used for CSC research. This medium does not contain serum, and is composed of recombinant stem cell growth protein, vitamins, minerals, etc., and small molecular compounds such as AMP, BMP inhibitor, Rock and SMOC-2 are added to inhibit the growth of miscellaneous cells and promote tumor formation of tumor stem cells.

Medium formula:

Basic medium: 250ml (P2400), cell growth factor (TSGCS) 25ml(P2402), G/A: 2.5ml (SD0038)

Note: 100ml bottle without kit, prepared for the entire bottle.

Note: The product is only used for scientific research

1. A nanotherapeutic system for gastric cancer suppression by synergistic chemotherapy and immunotherapy based on iPSCs and DCs exosomes.  Cancer Immunol Immunother 72, 1673–1683 (2023). 

https://doi.org/10.1007/s00262-022-03355-6

2. The ameliorative effect on chemotherapy-induced injury and tumor immunosuppressive microenvironment of the polysaccharide from the rhizome of Menispermum dauricum DC

https://doi.org/10.1016/j.ijbiomac.2024.131828

3. Xiaoli Wang, Yan Zhou, Xiaomin Lu, Lili Shao；Machine learning models reveal ARHGAP11A's impact on lymph node metastasis and stemness in NSCLC

published: 31 October 2024 https://doi.org/10.1002/biof.2141

4.Lei Shi，Jian Huang，Xifeng Fei，Bao He，Zhixiang Sun，guan sun，DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway，https://www.researchsquare.com/article/rs-5306321/v1

5.J. et al. DMC-BH derivative DMC-GF inhibits the growth of glioma stem cells by targeting the TRIM33/SLC25A1/mitochondrial oxidative phosphorylation pathway. J Transl Med 23, 363 (2025). https://doi.org/10.1186/s12967-025-06355-z

6.Licoisoflavone A inhibits colorectal cancer cell proliferation through targeting CDK2-Cyclin E1 axis-mediated cell cycle transition,https://doi.org/10.1016/j.bcp.2025.117124

7.Machine learning models reveal ARHGAP11A's impact on lymph node metastasis and stemness in NSCLC， https://doi.org/10.1002/biof.2141

8. Targeting HNRNPA2B1 to overcome chemotherapy resistance in gastric cancer stem cells: Mechanisms and therapeutic potential, https://doi.org/10.1016/j.jbc.2025.108234