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HL-1 (mouse cardiomyocytes)
No.:CD0686
Price: ¥1400.00
Products Description
Hl-1 myocardial cell line is a permanent mouse myocardial cell line, which can continuously divide and spontaneously contract while maintaining the differentiated cardiac phenotype. HL-1 can be continuously passaged without losing its differentiated cardiomyocyte phenotype, including morphological, biochemical and electrophysiological characteristics. HL-1 cells have been used to study the signal, electrical, metabolic and transcriptional regulatory functions of normal myocardial cells, and to solve pathological conditions, such as hypoxia, hyperglycemia-hyperinsulinemia, apoptosis and ischemia-reperfusion.
HL-1 is derived from AT-1 subcutaneous tumor of female adult C57BL/6J mice. The parent AT-1 was originally derived from the atrial tumor growing in transgenic mice. The expression of SV40 large t antigen targeted the atrial myocytes through the atrial natriuretic factor (ANF) promoter.
Organizational source/Issue and Cell Type: Heart
Morphology: Fibroblast like, adherent growth
Medium and Culture Conditions
90% MEM (MD0300)+10% fetal bovine serum (SD0200)+G/A (SD0038)
Complete medium: MEM complete medium (article number: MD0301)
Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.
Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)
Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)
Note: This product is for scientific research only
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