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  • SW480 [SW-480]人结肠癌细胞
  • SW480 [SW-480]人结肠癌细胞

SW480 [SW-480] Human Colon Cancer Cells

No.:CD0042

Price: ¥1400.00 ¥1700.00

specification:
1E+6 Cells Flask
amount: - +
  • SW480 [SW-480]人结肠癌细胞
  • SW480 [SW-480]人结肠癌细胞
Products Description

SW480 is derived from rectal adenocarcinoma in situ. SW620 ATCC CCL-227 originated from lymph node metastasis of the same patient one year later. CSA p and rectal antibody 3 were negative. Keratin immunoperoxidase staining was positive. The G ->A mutation at codon 273 of p53 gene causes Arg ->His substitution, and the C ->T mutation at codon 309 causes Pro ->Ser substitution. The expression level of p53 protein increased. The expression of oncogenes c-myc, K-ras, H-ras, N-ras, myb, sis and fos was positive. The expression of oncogene N-myc was not detected. It does not express cytolytic enzyme, a metalloproteinase associated with tumor invasion. It was reported that the cell expressed GM-CSF. There is a mutation in codon 12 of ras proto-oncogene, which can be used as a positive control for PCR detection of the mutation. When A. Leibovitz submitted it to ATCC in November 1978, it was passed down to the 91st generation.


Tissue and Cell Type: rectum; Colorectal Adenocarcinoma

Morphology: epithelial-like, adherent growth

Culture media and additives/Complete Growth Medium and Culture Conditions:

90% L-15 (MD1100)+10% fetal bovine serum (SD0200)+G/A (SD0038)

Complete medium: L-15 complete medium (article No.: MD1101)

Gas phase: air, 100%; Carbon dioxide, 0%. Temperature: 37 ℃.

Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)



Note: This product is for scientific research only


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