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RWPE-1 Human Normal Prostate Epithelial Cells
No.:CD0218
Price: ¥1600.00
Products Description
The epithelial cells in the surrounding area of normal prostate tissue section of a 54-year-old white man were transformed with a single copy of human papillomavirus 18 (HPV-18) to establish RWPE-1. It is reported that RWPE-1 cells form acini and secrete PSA into the culture medium under the stimulation of androgen when cultured in three-dimensional matrix. When mixed with matrigel or stroma cells to inject male nude mice, RWPE-1 cells can also form glandular cells and produce PSA. Cells derived from RWPE-1 were transfected with Ki-ras gene with Kirstin mouse sarcoma virus (Ki-MuSV) to establish tumorigenic RWPE-2 cells and RWPE2-W99 cells. In addition, RWPE-1 was treated with N-methyl-N-nitrourea (MNU) to establish a series of tumorigenic cell lines simulating different stages of prostate cancer progression. They are WPE1-NA22, WPE1-NB14, WPE1-NB11 and WPE1-NB26 cells. It is reported that RWPE-1 cells are negative for hepatitis B virus, hepatitis C virus and human immunodeficiency virus after detection. PCR confirmed that the HPV DNA sequence of the cell line was positive. It was identified as free of mycoplasma/STR and put into storage.
Tissue and Cell Type Prostate
Morphology epithelial cell-like, adherent growth
Culture media and additives/Complete Growth Medium and Culture Conditions:
Keratinocyte-SFM (Invitrogen, 17005-042)
K-SFM (Invitrogen, 17005-042) culture medium consists of two components: 1 bottle of basic culture medium (article No. 10724-011)+2 tubes of growth factor (article No. 37000-015)+1% GA (SD0038). According to the instructions of K-SFM culture solution, Gentamicin Solution needs to be added additionally
Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.
Complete cryopreservation solution: non-programmed cooling cell cryopreservation solution (low serum) (SD0001)
Note: This product is for scientific research only
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