Rat splenocytes-

Rat spleen is mechanically dissociated into a single cell suspension. The splenocytes are enriched by density separation. The rat splenocytes can be used to isolate CD4+ T cells, CD8+ T Cells, and CD45R+ B Cells, using specific immunomagnetic methods.

Medium Formulation:

RPMI-1640（MD0200）+10% FBS（SD0200）+2 mM L-谷氨酰胺（SD0007）+10 mM HEPES+P/ S（SD0005）

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only