v6.5V6.5 mouse embryonic stem cells-

The v6.5 mouse embryonic stem cell line was established from cells derived from the inner cell mass (ICM) of a 3.5 day old mouse embryo from a C57BL/6 X 129/sv cross and has been used successfully in producing germline transmitting chimeras. 

Medium Formulation:

MES complete culture solution formula (600 ml): DMEM (Invitrogen 12430) 497 ml ES grade FBS (biochrom S4115) 90 ml Glutamax (Invitrogen 35050) 6 ml NEAA (Invitrogen 11140) 6 ml LIF (Millipore ESG1107) 60 μ L (final concentration is 1000U/ml) β- Mer (Invitrogen 21985) 1.5 ml.

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only