-GFP Mouse cardiac microvascular endothelial cells GFP-

Mouse Heart Microvascular Endothelial cells (mHtMECs) are isolated from hearts of 3-week old male C57BL/6 mice and GFP expressing mHtMECs-GFP were selected from puromycin resistant cells after transfected with lentiviral expressing GFP. Cells are shipped in a frozen vial (provided @ passage 3, with > 5 x 105 cells/vial). mHtMECs-GFP are cultured with specially formulated Endothelial Growth Medium. Since the cells have limited proliferation capacity (cells may be expanded for a maximum of 2-3 passages) in vitro, long term culture of mHtMECs is not recommended, even cultured following the detailed protocol described below.

Medium Formulation:

ECGM endothelial cell culture medium (P1001)

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS (SD0200)

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.

For research use only