T-- (HTC) -

Human T Cells-Negatively Selected (HTC) are purified from healthy donors and ready to use in studies of T cell biology. HTC can respond to mitogens or anti-CD3 antibodies with robust proliferation and secretion of IFN, tumor necrosis factor  and interleukin-6. HTC are cryopreserved immediately after isolation and purification to ensure preservation of all circulating T cell subpopulations and antigen specificities. Our HTC are quality tested via flow cytometry to ensure depletion on undesired cell types and are typically 80% CD3 positive. HTC are positive for CD3 and contain both CD4 and CD8 subsets. HTC are negative for other lineage markers such as CD14, CD19 and CD56.

Medium Formulation:

RPMI-1640 Basic Medium（MD0200）+10%FBS（SD0200）+1%GA(SD0038)

Complete medium:

RPMI-1640 Complete Medium（MD0227）

Gas phase: air, 95%; Carbon dioxide, 5%. Temperature: 37 ℃.

Freezing conditions: basic medium+5% DMSO+20% FBS

Complete cryopreservation solution: No Programmed Cryopreservation Cells Solution (low Serum)(SD0001)

Declaration: This product is for scientific research only.